General description
The FLAG epitope, is an eight amino acid protein with an enterokinase-cleavage site.
The rabbit Anti-FLAG polyclonal affinity antibody ANTI-FLAG recognizes the FLAG epitope located on FLAG fusion proteins. This antibody reacts with N-terminal, N-terminal-Met, and C-terminal FLAG fusion proteins.
Immunogen
FLAG; peptide sequence DYKDDDDK
Application
The antibody recognizes the FLAG epitope located on FLAG-tagged fusion proteins at the N-terminus or C-terminus, applying dot blot, immunoblotting, immunoprecipitation and immunocytochemistry assays.
Browse additional application references in our FLAG® Literature portal."
Biochem/physiol Actions
FLAG epitope plays a crucial role in immunoaffinity purification of fusion proteins.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide
Preparation Note
Purified by affinity chromatography on a column bearing the immunizing peptide.
Legal Information
FLAG is a registered trademark of Sigma-Aldrich Co. LLC
- UPC:
- 41181506
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- F7425-.2MG
