ANTI-FLAG M2 AFFINITY GEL (C005B-238818)

General description

Anti-FLAG M2 Affinity gel is a mouse monoclonal antibody that is covalently attached to agarose. The antibody binds FLAG at the N-terminal, Met-N-terminal, C-terminal and internal locations of fusion proteins. Binding is calcium-independent.

Elution - FLAG^® peptide, Glycine, pH 3.5, 3x FLAG^® peptide

Immunogen

DYKDDDDK

Application

Anti-FLAG^® M2 affinity gel has been used for western blotting, immunoprecipitation and for the purification of FLAG fusion proteins.

Browse additional application references in our FLAG^® Literature portal.

Physical form

Suspension in buffered saline containing azide as preservative and 50% glycerol

Legal Information

ANTI-FLAG is a registered trademark of Sigma-Aldrich Co. LLC

FLAG is a registered trademark of Sigma-Aldrich Co. LLC

conjugate: agarose conjugate. Quality Level: 200. antibody form: purified immunoglobulin. antibody product type: primary antibodies. clone: M2, monoclonal. form: buffered aqueous glycerol solution. analyte chemical class(es): proteins (FLAG^®. tag, 3x FLAG^®. , DYKDDDDK tag). technique(s): affinity chromatography: suitable (FLAG^®. peptide, Glycine, pH3.5, 3x FLAG^®. peptide), immunoprecipitation (IP): suitable. matrix: (4% agarose bead. 45-165μ. m bead size). isotype: IgG1. capacity: >. 0.6 . mg/mL, resin binding capacity (FLAG-BAP). web metadata keyword.default: ANTI-FLAG M2 Affinity Agarose Gel, Monoclonal ANTI-FLAG M2 antibody produced in mouse, FLAG Detect, FLAGdetect, FLAGcapture, FLAGfusion, FLAGantibody, FLAGselect, epitope, monoclonal, M2, Affinity Gel, anti-Flag, DYKDDDDK, octapeptide, recombinant, peptide. shipped in: wet ice. storage temp.: −. 20°C. Storage Class Code: 10 - Combustible liquids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.

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