General description

RNA binding protein fox-1 homolog 1 (UniProt Q9NWB1; also known as Ataxin-2-binding protein 1, Fox-1 homolog A, Hexaribonucleotide-binding protein 1) is encoded by the RBFOX1 (also known as A2BP, A2BP1, FOX1, HRNBP1) gene (Gene ID 54715) in human. The RNA-binding Fox (Rbfox) family of splicing factors is comprised of three members, Rbfox1 (Fox-1 or A2BP1), Rbfox2 (Fox-2 or RBM9), and Rbfox3 (Fox-3, HRNBP3 or NeuN). Rbfox proteins regulate splicing of many neuronal transcripts (pre-mRNAs) by binding the sequence (U)GCAUG in introns flanking alternative exons. A (U)GCAUG motif that lies downstream of the alternative exon generally promotes Rbfox-dependent exon inclusion, whereas an upstream motif will usually repress exon inclusion. Rbfox1 transcript itself is subjected to alternative splicing in response to chronic cell depolarization, resulting in the increased expression of the nuclear, splicing-active Rbfox1 isoform, which in turn leading to increased splicing of Rbfox1 target transcripts of proteins, including N-methyl D-aspartate (NMDA) receptor 1 (Grin1) and a calcium ATPase (Atp2b1), that play important roles in neuronal excitation and calcium homeostasis.

Specificity

This monoclonal antibody immunostained exclusively differentiated neurons, but not neural stem cells, neuronal progenitors, astrocytes, or oligodendrocytes derived from p53-/-Pten+/- murine premalignant neural/progenitor stem cell (M-NSC) cultures (Hu, J., et al. (2013). Proc. Natl. Acad. Sci. U.S.A. 110(36):14520-14527).

Immunogen

Recombinant full-length human Fox1.

Application

Anti-Fox1, clone A2BP1, Cat. No. MABE960, is a highly specific rat monoclonal antibody that targets Fox1 and has been tested in Immunocytochemistry, Immunofluorescence, and Immunohistochemistry.

Immunohistochemistry Analysis: A 1:50-1,000 dilution from a representative lot detected Fox1 in mouse cerebral cortex and heart tissue sections.

Immunocytochemistry Analysis: A representative lot immunostained exclusively differentiated neurons, but not neural stem cells, neuronal progenitors, astrocytes, or oligodendrocytes derived from p53-/-Pten+/- murine premalignant neural/progenitor stem cells (M-NSCs) cultured under various differentiation conditions (Hu, J., et al. (2013). Proc. Natl. Acad. Sci. U.S.A. 110(36):14520-14527).

Immunofluorescence Analysis: A representative lot immunostained postmitotic terminally differentiated neurons in brain sections from 2-week old postnatal mice (Hu, J., et al. (2013). Proc. Natl. Acad. Sci. U.S.A. 110(36):14520-14527).

Immunohistochemistry Analysis: A representative lot detected downregulated Fox1 immunoreactivity in more than 90% of human GBM tissue samples when compared with normal human brain tissues (Hu, J., et al. (2013). Proc. Natl. Acad. Sci. U.S.A. 110(36):14520-14527).

Research Category
Epigenetics & Nuclear Function

Quality

Evaluated by Immunohistochemistry in human cerebellum tissue section.

Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected Fox1 in human cerebellum tissue section.

Target description

42.78/44.79/40.01/42.08/42.40 kDa (human isoform 1/2 (gamma)/3/4 (alpha)/5 (beta)) and 42.68/40.49/44.73/40.71/40.29/42.34/38.92 kDa (mouse isoform 1 (A016)/2/3 (A713)/4/5 (A030)/6 (A715)/7 (A704)) calculated.

Physical form

Format: Purified

Protein G purified.

Purified rat IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.