Anti-FoxP1 Antibody (C15-1317-217)

General description

Forkhead Box P1 (FoxP1) is a member of the FOX family of transcription factors. This protein is known to forms homodimers and heterodimers with family members FoxP2 and FoxP4. The forkhead box family has a broad range of functions. These proteins play important roles in immune response, organ development and the development of cancer. The FoxP1 protein is widely expressed and contains both forkhead (winged-helix) and C2H2 zinc finger nucleic acid-binding motifs. At least four different isoforms that result from alternative splicing of the mRNA transcript have been reported.

Specificity

This antibody recognizes FoxP1.

Immunogen

Epitope: Unknown

GST-tagged recombinant protein corresponding to mouse FoxP1.

Application

Immunocytochemistry Analysis: 1:500 dilution from a representative lot detected FoxP1 in HeLa and NIH/3T3 cells.

Immunohistochemistry Analysis: A representative lot was used by an independent laboratory in IH. (Lu, M.M., et al. (2002). Mechanisms of Development 119S:S197–S202.)

Research Category
Epigenetics & Nuclear Function

Epigenetics & Nuclear Function

Research Sub Category
Transcription Factors

Developmental Signaling

Use Anti-FoxP1 Antibody (Rabbit Polyclonal Antibody) validated in ICC, WB, IHC to detect FoxP1 also known as forkhead box P1, fork head-related protein like B, glutamine-rich factor 1.

Quality

Evaluated by Western Blot in RAW264.7 cell lysate.

Western Blot Analysis: 1:1,000 dilution of this antibody detected FoxP1 on 10 µg of RAW264.7 cell lysate.

Target description

79 kDa observed. Alternative splicing can result in cell type dependent isoforms.

Physical form

Unpurified

Unpurified rabbit polyclonal serum with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Analysis Note

Control
RAW264.7 cell lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. Quality Level: 100. antibody form: serum. antibody product type: primary antibodies. clone: polyclonal. species reactivity: human, mouse. technique(s): immunocytochemistry: suitable, immunohistochemistry: suitable, western blot: suitable. isotype: IgG. NCBI accession no.: NP_444432. UniProt accession no.: P58462. shipped in: wet ice. target post-translational modification: unmodified. Gene Information: human ... FOXP1(27086). Storage Class Code: 10 - Combustible liquids. WGK: WGK 1.