ANTI-FOXP2 ANTIBODY CLONE FOXP2-73A/8

General description

Forkhead box protein P2 (FoxP2) is a multidomain transcriptional repressor that belongs to the Fox family of proteins. The FoxP2 protein contains a forkhead-box DNA-binding domain, characteristic of the FOX group of transcription factors. In addition, this protein contains a polyglutamine tract, as well as zinc finger and leucine zipper motifs. In order for FoxP2 to bind DNA, it forms homodimers and heterodimers with FoxP1 and FoxP4. The forkhead box family of proteins play an important role in immune response, organ development and the development of cancer. Functional studies in vertebrates suggest this gene plays a role in the modulation of neural plasticity. In line with these findings, mutations of FoxP2 in humans have been linked to speech and language disorders.

Specificity

This antibody recognizes FoxP2 isoforms at 70 kDa, 48 kDa, and 40 kDa.

Immunogen

GST-tagged recombinant protein corresponding to the N-terminus of Human FoxP2.

Application

Detect FOXP2 using this mouse monoclonal antibody, Anti-FoxP2 Antibody, clone FOXP2-73A/8 validated for use in western blotting & IHC.

Western Blotting Analysis: 1 µg/mL from a representative lot detected FoxP2 in 10 µg of THP-1 cell lysate.

Immunoprecipitation Analysis (IP): A representative lot of this antibody was demonstrated to immunoprecipitate FoxP2 using growth arrested 143B cells (Gascoyne D.M., et al. (2015). PLoS One, 10(6):e0128513).

Immunohistochemistry Analysis: A representative lot from an independent laboratory detected FoxP2 in human tonsil tissues (Campbell, A. J., et al. (2010). Br J Haematol. 149(2):221-230.).

Immunohistochemistry Analysis: A representative lot from an independent laboratory detected FoxP2 in proliferating chondrocytes of murine E17.5 long bone (Gascoyne D.M., et al. (2015). PLoS One, 10(6):e0128513).

Quality

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected FoxP2 in 10 µg of HeLa cell lysate.

Target description

~80 kDa observed. Uniprot describes 9 isoforms between 9-83 kDa

Physical form

Format: Purified

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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