ANTI-FOXP4 ANTIBODY (C005B-241033)

General description

Forkhead box protein P4 (FoxP4) is a multidomain transcriptional repressor. This protein is a member of the Fox family of proteins characterized by it unique winged-helix DNA binding motif. Members of this family are known for their ability to regulate both the cell fate and differentiation of several tissues. FoxP4 acts to repress expression by inteacting with DNA. For DNA binding to occur, FoxP4 must form homodimers and heterodimers with FoxP1 and FoxP2. During embryogenesis, expression of this protein is observed in lung and brain. In adults expression is observed in adult heart, lung, brain, liver, spleen, testis and kidney. Alternative splicing result in the expression of three known isoforms.

Specificity

This antibody recognizes FoxP4.

Immunogen

Epitope: Unknown

GST-tagged recombinant protein corresponding to mouse FoxP4.

Application

Immunocytochemistry Analysis: 1:500 dilution from a representative lot detected FoxP4 in HeLa cells.

Immunohistochemistry Analysis: A representative lot was used by an independent laboratory in IH. (Lu, M.M., et al. (2002). Mechanisms of Development 119S:S197–S202.)

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Transcription Factors

Use Anti-FoxP4 Antibody (Rabbit Polyclonal Antibody) validated in ICC, WB, IHC to detect FoxP4 also known as forkhead box P4, winged-helix repressor FOXP4.

Quality

Evaluated by Western Blot in human brain cell lysate.

Western Blot Analysis: 1:1,000 dilution of this antibody detected FoxP4 on 10 µg of human brain cell lysate.

Target description

86 and 79 kDa Observed. Three known isoforms due to alternative splicing.

Physical form

Unpurified

Unpurified rabbit polyclonal serum with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Analysis Note

Control
Human brain cell lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. Quality Level: 100. antibody form: serum. antibody product type: primary antibodies. clone: polyclonal. species reactivity: human. species reactivity (predicted by homology): mouse (based on 100% sequence homology). technique(s): immunocytochemistry: suitable, immunohistochemistry: suitable, western blot: suitable. NCBI accession no.: NP_001104294. UniProt accession no.: Q9DBY0. shipped in: wet ice. target post-translational modification: unmodified. Gene Information: mouse ... Foxp4(74123). Storage Class Code: 10 - Combustible liquids. WGK: WGK 1.

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