Specificity
GABA (gamma-aminobutyrate). The antibody has been calibrated against a spectrum of antigens to assure hapten selectivity and proper affinity. No measurable glutaraldehyde-fixed tissue cross-reactivity (<1:1000) against L-alanine, 1-amino-4-guanidobutane (AGB), D/L-arganine, D/L-aspartate, L-citrulline, L-cysteine, D/L-glutamate, D/L-glutamine, glutathione, glycine, L-lysine, L-ornithine, L-serine, L-threonine, L-tryptophan, L-tyrosine.
Immunogen
GABA-glutaraldehyde
Application
Immunohistochemistry using silver-intensified immunogold or fluorescence (see recommended protocol). Samples should be fixed with 0.05% - 2.5% glutaraldehyde for optimum detection.
*This antibody has also been used and found to work with a zero-low glutaraldehyde / high paraformaldehyde fixation (4% paraformaldehyde in 0.1M phosphate buffer / 3% sucrose fixative ). The minimum glutaraldehyde concentration for AB5016 is 0.00%. See protocol that follows. Performance is good with frozen sections, Vibratome sections and tissue culture formats, when penetrating reagents such as 0.3% Triton X-100 are used.
Optimal working dilutions must be determined by the end user.
DILUTION: Prepare enough of the AB5016 for your days use by diluting 100X with 1% GSPBT.
Research Category
Neuroscience
Research Sub Category
Neurotransmitters & Receptors
This Anti-GABA (No Gluteraldehyde) Antibody is validated for use in IH for the detection of GABA (No Gluteraldehyde).
Physical form
Format: Purified
IgG fraction in sterile 0.1M phosphate buffer. No preservative
Storage and Stability
Maintain stock at 2-8°C in undiluted aliquots for up to 6 months. This stock is extremely stable under normal use and routine storage at 2-8°C. Do not freeze this stock.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
biological source: rabbit. Quality Level: 100. antibody form: purified immunoglobulin. antibody product type: primary antibodies. clone: polyclonal. species reactivity: rat. manufacturer/tradename: Chemicon®. . technique(s): immunohistochemistry: suitable. shipped in: wet ice. target post-translational modification: unmodified. Gene Information: rat ... Gabra1(29705).- UPC:
- 51201516
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- AB5016