ANTI-GAL4 AD (ACTIVATION DOMAIN) AFFINITY ISOLATED RABBIT ANTIBODY

General description

GAL4 protein is an 881 amino acid transcription factor containing an independent N-terminal DNA binding (147 amino acids) and C-terminal activator domains. It is a regulatory gene that belongs to GAL gene family. It is a component of yeast two hybrid system, where GAL 4 DNA-BD is fused to protein X (bait) and GAL4 activation domain is fused to protein Y (prey).

Useful in the study of protein:protein interaction studies. GAL4 DNA activation domain fusion proteins.

Immunogen

Synthetic peptide corresponding to amino acids of Saccharomyces cerevisiae GAL4 protein.

Application

Anti-GAL4, Activation domain antibody produced in rabbit has been used in western blotting.

Biochem/physiol Actions

GAL4 induces the genes that are responsible for regulation of galactose metabolism in saccharomyces cerevisiae. GAL4 protein is widely used for studying transcriptional activation in eukaryotes. The yeast two hybrid system is widely used to study to discover protein− protein interactions (PPIs) and protein−DNA interactions.

Physical form

The product is provided as a solution of affinity isolated antibody in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide as a preservative.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. Storage in “frost-free” freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.