ANTI-GAL4 DNA-BD AFFINITY ISOLATED RABBIT ANTIBODY

General description

The polyclonal Anti-GAL4 DNA-BD rabbit antibody is a synthetic peptide that recognizes GAL4 DNA binding domain fusion proteins. GAL4 protein is an 881 amino acid transcription factor. It contains functionally independent N-terminal DNA binding (147 amino acids) and C-terminal activator domains. It is a constituent of yeast two hybrid system, where GAL 4 DNA-BD is fused to protein X (bait) and GAL4 activation domain is fused to protein Y (prey).

Specificity

Anti-GAL4 DNA-BD recognizes GAL4 DNA binding domain fusion proteins.

Immunogen

synthetic peptide corresponding to the Saccharomyces cerevisiae GAL4 protein DNA binding domain (amino acids 39-52).

Application

Anti-GAL4 DNA-BD antibody produced in rabbit has been used in western blotting.

Biochem/physiol Actions

GAL4 transcription factor is involved in the induction of genes that regulate galactose metabolism in Saccharomyces cerevisiae. The yeast two hybrid system is widely used for the characterization of domains that are necessary and sufficient for the interaction of two known proteins by deletion and mutational analysis.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Storage and Stability

For continuous use store at 2-8°C for up to one month. For extended storage, freeze in working aliquots at –20 °C. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.