ANTI-GALACTOCEREBROSIDE ANTIBODY CLONE MGALC

General description

Galactocerebroside (GalC) is a major galactosphingolipid of myelin which plays a role in myelination. GalC is a very useful, specific marker for oligodendroglial lineage.

Specificity

Galactocerebroside (GalC), sulfatide, psychosine and other galactolipids. Cross-reacts with the sulfatide ester of GalC, but to a 16-fold lesser extent. No cross-reactivity with sphingosine, ceramide, mixed ganglioside or glucocerebroside. Binds specifically with oligodendrocytes and Schwann cells.

Immunogen

Synaptic plasma membranes from bovine hippocampus. Ranscht, B. et al. PNAS 79(8):2709-2713 (1982)

Application

Anti-Galactocerebroside Antibody, clone mGalC is an antibody against Galactocerebroside for use in ELISA, IC, & IHC.

Immunohistochemistry:
0.5-10 μg/mL of a previous lot was used on formalin fixed frozen sections of a prevous lot. Can not be used on paraffin embedded tissue sections since the antigen is denatured during embedding and paraffin removal.

Immunocytochemistry:
0.5-10 μg/mL of a previous lot was used on 4% paraformaldehyde, acetic acid or ethanol fixed cultured cells.

ELISA:
A previous lot was used on purified galactocerebrosides.

Optimal working dilutions must be determined by the end user.

Research Category
Neuroscience

Research Sub Category
Neuronal & Glial Markers

Physical form

Format: Purified

Protein A purified

Purified mouse monoclonal in buffer containing 10 mM Potassium Phosphate, 150 mM NaCl, pH 7.4 containing 0.09% sodium azide.

Storage and Stability

Stable for 1 year at -20ºC from date of receipt.

Analysis Note

Control
Neonatal cortex

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.