Anti-Galactocerebroside antibody produced in rabbit

General description

Galactocerebroside is the major galactosphingolipid of myelin. It is expressed in the CNS in cell membranes of oligodendrocytes. It is also expressed in Schwann cells in peripheral nerves.

This product is a pooled delipidized antiserum produced in rabbits by repeated injections of a mixture of galactocerebrosides I and II conjugated to keyhole limpet hemocyanin.

Specificity

The antibody localizes oligodendroglia in cultured cells. It may be used for studying changes in the organization of oligodendroglial membrane sheets in culture. Specificity of the product is determined by dot blotting using galactocerebroside as the antigen.

Immunogen

Galactocerebrosides (GalC) I and II conjugated to KLH

Application

Anti-Galactocerebroside antibody produced in rabbit has been used:

• for dot-blot immunoassay and western blot analysis of mouse brain homogenates
• as a primary antibody at a working dilution of 1:50 for immunostaining of muscle tissue of Rana pipiens frogs
• as a primary antibody at a working dilution of 1:500 in immunofluorescence and immunohistochemistry using primary human astroglia

Biochem/physiol Actions

Galactocerebroside is the major galactosphingolipid of myelin. It is expressed in the CNS in cell membranes of oligodendrocytes. It is also expressed in Schwann cells in peripheral nerves. Galactocerebroside is found in serum and cerebral spinal fluid of patients with demyelinating diseases like multiple sclerosis.

Galactocerebroside was shown to play an important role in the processes of normal development and myelination and is known to be an early marker of oligodendrocyte development.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.