Anti-GATA1 antibody produced in rabbit (C15-1425-530)

General description

GATA is an important cis-element factor in the haematopoietic lineage belongs to GATA-binding protein family. It was first identified in the analysis of globin gene promoters and enhancers. There are different types of GATA proteins: GATA-1, GATA-2, GATA-3. It is expressed in erythroid, mast, megakaryocytic lineages, multipotential progenitor cells and lines.

GATA-1 (ERYF1, GF-1, NF-1) is a Cys2/Cys2 zinc finger DNA binding protein that is expressed primarily in erythroid, megakaryocytic, mast cells and eosinophilic cells.

Immunogen

synthetic peptide corresponding to the C-terminal region of human GATA-1 (amino acids 394-413).

Application

Anti-GATA1 antibody produced in rabbit has been used in gel mobility shift assay and DNase I footprinting. It has also been used in confocal microscopy analysis.

Biochem/physiol Actions

GATA is a lineage-restricted transcription factor which plays a pivotal role in erythroid differentiation. It possesses potential binding sites in the regulatory sequences of all erythroid genes. GATA is expressed at higher levels during erythroid maturation. It is essential for terminal differentiation of erythrocytes and megakaryocytes and associated in vivo with the acetyl transferase p300/CBP. Mutation in GATA1 causes thrombocytopenia and abnormal platelet function.

GATA-binding factor 1 (GATA-1) is involved in blocking apoptosis of precursor cells and in controlling the balance between proliferation and cell cycle arrest.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.