Anti-Gbeta5 Antibody (C15-1317-692)

General description

The Gβ5 (GNB5 guanine nucleotide binding protein (G protein), beta 5) is a G protein subunit present predominantly in the central nervous system. This protein appears to be expressed in two forms, one found in the brain of 39 kDa and one found in the retina of 44 kDa. It has also been found that Gβ5 associates tightly with RGS7, a member of the superfamily of negative regulators of G protein signaling. These complexes have been localized to most regions of the brain, whereas Gβ1-4 exhibit no observable colocalization with RGS7, despite their widespread codistribution throughout similar neuronal networks. This antibody is specific for a peptide sequence within the D1 blade which is not found in Gβ1-4.

Immunogen

Epitope: C-terminus

KLH-conjugated linear peptide corresponding to the C-terminus of human Gβ5.

Application

Anti-Gβ5 is an antibody against Gβ5 for use in WB, ICC, IF.

Research Category
Signaling

Research Sub Category
Signaling Neuroscience

Western Blotting Analysis: A representative lot detected Gβ5 in HA-ERK2, HA-MAPK, and Gβ5 in cotransfected COS cells, cholate membrane extracts of control (Con) or transfected (b5 1 g2) COS cells, mouse, or bovine brain (Zhang, S., et al. (1996). JBC. 271(52):33575-33579).
Western Blotting Analysis: A representative lot detected Gβ5 in PC12 and mouse brain, and PC12 cells transiently transfected with GFP fusion constructs (Zhang, S., et al. (2001). JBC. 276(13):10284-10289).
Western Blotting Analysis: A representative lot detected Gβ5 in PC12 transfected with fusion proteins, brains of wild-type, R7 binding protein (R7BP), and knockout (KO) mice (Leelamma, M.P., et al. (2010). Journal of Neurochemistry. 113:1101-1112).
Immunocytochemistry Analysis: A representative lot detected Gβ5 in NGF-differentiated PC12 cells, NGF-differentiated PC12 cells stably transfected with pTREHAGb5 encoding HA epitope-tagged Gb5 were induced with doxycycline (Zhang, S., et al. (2001). JBC. 276(13):10284-10289).
Immunocytochemistry Analysis: A representative lot detected Gβ5 in PC12 cells transfected with the cDNA
combinations HA-Gb5/RGS7HA-Gb5/DRGS-RGS7, or HA-Gb5/Depless-RGS7, Neuro-2A or SH-SY5Y cells transfected with the HAGb5/RGS7 or HA-Gb5/Depless-RGS7 (Leelamma, M.P., et al. (2010). Journal of Neurochemistry. 113:1101-1112).
Immunofluorescence Analysis: A representative lot detected Gβ5 in primary cortical neuronal cells derived from wild-type and R7 binding protein (R7BP) homozygous knockout (KO) mice (Leelamma, M.P., et al. (2010). Journal of Neurochemistry. 113:1101-1112).

Quality

Evaluated by Western Blotting in mouse brain tissue lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected Gβ5 in 10 µg of mouse brain tissue lysate.

Target description

~39 kDa observed. Uniprot describes 3 isoforms produced by alternative splicing at ~44 kDa, ~39 kDa, and ~31 kDa and this antibody is predicted to cross-react with all three.

Physical form

Rabbit polyclonal serum with 0.05% sodium azide.

Unpurified

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. Quality Level: 100. antibody form: serum. antibody product type: primary antibodies. clone: polyclonal. species reactivity: rat, mouse. species reactivity (predicted by homology): human (based on 100% sequence homology), feline (based on 100% sequence homology), canine (based on 100% sequence homology), bovine (based on 100% sequence homology). technique(s): immunocytochemistry: suitable, immunofluorescence: suitable, western blot: suitable. NCBI accession no.: NP_0057287. UniProt accession no.: O14775. shipped in: dry ice. target post-translational modification: unmodified. Gene Information: dog ... Gnb5(608623)
human ... GNB5(10681)
mouse ... Gnb5(14697)
rat ... Gnb5(83579). Storage Class Code: 10 - Combustible liquids. WGK: WGK 1.