ANTI-GFP N-TERMINAL DEVELOPED IN RABBIT AFFINITY ISOLATED ANTIBODY

General description

Green fluorescent protein (GFP) chromophore contains hexapeptide fragment with Phe64-Ser-Tyr-Gly-Val-Gln69 aminoacid sequence. GFP structure contains a 11-stranded β-barrel and a central helix and corresponds to a molecular weight of 26 kDa.

Specificity

Specific for GFP fusion proteins.

Immunogen

synthetic peptide corresponding amino acids 3-17 of GFP.

Application

Anti-GFP, N-terminal antibody produced in rabbit has been used:

• in immunoblotting
• in the histochemistry and immunohistochemistry
• in the immunoprecipitation

Biochem/physiol Actions

Green fluorescent protein (GFP) has photochemical and diagnostic applications. It is also used widely in protein dynamics studies. The polyclonal anti-GFP, N-terminal rabbit antibody reacts specifically with GFP fusion proteins.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.