Anti-Gialpha-1-Subunit, Internal (159-168) Rabbit pAb (C15-1303-083)

General description

Anti-Giα-1-Subunit, Internal (159-168), rabbit polyclonal, recognizes Giα-1 subunit. Does not cross-react with Giα-2, Giα-3, Goα, or Gsα subunits. It is validated for use in Western blotting.

Rabbit polyclonal antibody supplied as diluted serum. Recognizes G_iα-1 subunit.

Recognizes G_iα-1 subunit. Does not cross-react with G_iα-2, G_iα-3, G_oα, or G_sα subunits.

Immunogen

a synthetic peptide (LDRIAQPNYI) corresponding to amino acids 159-168 of mouse G_iα-1, conjugated to KLH

Application

Immunoblotting (1:1000)

Warning

Toxicity: Standard Handling (A)

Physical form

Serum diluted in 140 mM NaCl, 100 mM potassium phosphate, pH 7.5.

Reconstitution

Following initial thaw, aliquot and freeze (-70°C).

Other Notes

Does not cross-react with G_iα-2, G_iα-3, G_oα, or G_sα subunits. The specificity and cross-reactivity were confirmed with lysates from separate cultures of bacteria transfected with the genes for G_iα-1, G_iα-2, G_iα-3, G_oα and G_sα. Variables associated with assay conditions will dictate the proper working dilution.

Kumar, R., et al. 1994. J. Mol. Cell. Cardiol.26, 1537.
Mumby, S.M., and Gilman, A.G. 1991. Methods Enzymol.195, 215.
Goldsmith, P.P., et al. 1987. J. Biol. Chem.262, 14683.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

biological source: rabbit. Quality Level: 100. antibody form: diluted serum. antibody product type: primary antibodies. clone: polyclonal. form: liquid. does not contain: preservative. species reactivity: mouse. manufacturer/tradename: Calbiochem^®. . storage condition: OK to freeze, avoid repeated freeze/thaw cycles. isotype: IgG. shipped in: wet ice. storage temp.: −. 70°C. target post-translational modification: unmodified. Gene Information: mouse ... Gnai1(14677). Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.