ANTI-GIRDIN ANTIBODY (C15-1318-008)

General description

Girdin (Ccdc88a; APE, GIV, or HkRP1) is a ubiquitous protein belonging to the CCDC88 family. Girdin is an actin-binding protein which interacts with actin via its C-terminal domain, and may play a crucial role in cell motility and cytoskeleton remodeling. These effects may be regulated by a reciprocal relationship between Girdin and Akt. Akt phosphorylates Girdin, and Girdin binds to Akt and enhances phosphorylation of Akt, thereby promoting phosphorylation of Akt’s downstream targets such as GSK-3β and FOXO1A. Girdin/Akt signaling may play an important role in cancer by facilitating the proliferation of cancer cells.

Specificity

Other homologies: Mouse (97% sequence homology).

This antibody recognizes the coiled-coil domain of Girdin.

Immunogen

Epitope: Coiled coil domain.

GST-tagged recombinant protein corresponding to the coiled coil domain rat Girdin.

Application

Immunohistochemistry Analysis: A 1:2,000 dilution from a representative lot detected Girdin in human colorectal cancer tissues.

Immunofluorescence Analysis: A 1:500 dilution from a representative lot detected Girdin in HeLa, Cos-7, and Att20 cells.

Immunoprecipitation Analysis: A representative lot immunoprecipitated Girdin in HeLa cell lysate.

Research Category
Cell Structure

Research Sub Category
Cytoskeleton

This Girdin antibody is validated for use in WB, IHC, IF & IP for the detection of the Girdin protein.

Quality

Evaluated by Western Blotting in Cos-7 cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected Girdin in 10 µg of Cos-7 cell lysate.

Target description

~220 kDa observed. Uncharacterized band(s) may be observed in some cell lysates.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Cos-7 cell lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. Quality Level: 100. antibody form: affinity isolated antibody. antibody product type: primary antibodies. clone: polyclonal. purified by: affinity chromatography. species reactivity: human, monkey. species reactivity (predicted by homology): rat (immunogen homology). technique(s): immunofluorescence: suitable, immunohistochemistry: suitable (paraffin), immunoprecipitation (IP): suitable, western blot: suitable. NCBI accession no.: XP_223709. shipped in: wet ice. target post-translational modification: unmodified. Gene Information: human ... CCDC88A(55704). Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.