General description
Glial fibrillary acidic protein is a class-III intermediate filament. GFAP is the main constituent of intermediate filaments in astrocytes and serves as a cell specific marker that distinguishes differentiated astrocytes from other glial cells during the development of the central nervous system.
Specificity
It is expected that the antibody will react with most mammalian GFAP due to homology. Reactivity with other species has not been confirmed.
Recognizes Glial Fibrillary Acidic Protein (GFAP).
Immunogen
Purified bovine GFAP
Application
Anti-Glial Fibrillary Acidic Protein (GFAP) Antibody is an antibody against Glial Fibrillary Acidic Protein (GFAP) for use in IC, IH, IH(P) with more than 65 product citations.
Immunohistochemistry(paraffin):
Representative images from a previous lot.
Immunocytochemistry:
A 1:1,000 dilution of a previous lot was used in Immunocytochemistry.
Research Category
Neuroscience
Research Sub Category
Neuronal & Glial Markers
Quality
Routinely evaluated by immunohistochemistry on brain tissue, astrocytes, and neurons.
Immunohistochemistry(paraffin) Analysis:
GFAP (IHC2078) staining of Human Brain. Tissue pretreated with Citrate Buffer, pH 6.0. Pre-diluted polyclonal antibody, using IHC-Select
Optimal Staining with Citrate Buffer Epitope Retrieval: Human Brain
Target description
50 kDa
Linkage
Replaces: AB1540
Physical form
Rabbit serum. Liquid. Contains no preservative.
Unpurified
Storage and Stability
Stable for 1 year at -20°C undiluted aliquots from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Analysis Note
Control
Mouse brain tissue, rat astrocytes, rat neurons.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
biological source: rabbit. Quality Level: 100. antibody form: serum. antibody product type: primary antibodies. clone: polyclonal. species reactivity: human, bovine, canine, rat. manufacturer/tradename: Chemicon®. . technique(s): immunocytochemistry: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, western blot: suitable. NCBI accession no.: NM_002055.2. UniProt accession no.: Q28115. shipped in: dry ice. target post-translational modification: unmodified. Gene Information: human ... GFAP(2670). Storage Class Code: 10 - Combustible liquids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.- UPC:
- 12352202
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- AB5804