Anti-GLS (C15-1317-375)

General description

Glutaminase kidney isoform, mitochondrial (UniProt: O94925; also known as EC:3.5.1.2, GLS, K-glutaminase, L-glutamine amidohydrolase) is encoded by the GLS (also known as GLS1, KIAA0838) gene (Gene ID: 2744) in human. GLS catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. It is primarily expressed in the brain and kidney and plays an essential role in generating energy for metabolism, synthesizing the brain neurotransmitter glutamate and maintaining acid-base balance in the kidney. During normal acid-base balance, the kidney extraction of plasma glutamine is low, however, during metabolic acidosis, about one third of the plasma glutamine is extracted and metabolized in a single pass through kidneys. Three isoforms of glutaminase have been described in humans. They are produced via alternate splicing. Isoform 1 and 3 are activated by phosphate, while the isoform 3 lacks catalytic activity. Isoform 1 is highly expressed in brain and kidney, but is not detected in liver. On the other hand, isoform 3 is highly expressed in astrocytoma, ganglioglioma, ependymoma. Lower levels of isoform 3 are reported in placenta, lung, pancreas and kidney, but it is not detected in liver. Isoform 2 is expressed in cardiac and skeletal muscle.

Specificity

This polyclonal antibody detects the Glutaminase kidney isoform, mitochondrial, in human. Also detects GLS in the neural cortex. It targets an epitope within 16 amino acids fromt he C-terminal region.

Immunogen

KLH-conjugated linear peptide corresponding to 16 amino acids from the C-terminal region.

Application

Anti-GLS, Cat. No. ABN1466, is a highly specific rabbit polyclonal antibody that targets Glutaminase kidney isoform, mitochondrial, and has been tested in Western Blotting.

Research Category
Neuroscience

Western Blotting Analysis: 2 µg/mL from a representative lot detected GLS in 10 µg of human kidney tissue lysate.

Quality

Evaluated by Western Blotting in human neural cortex tissue lysate.

Western Blotting Analysis: 2 µg/mL of this antibody detected GLS in 10 µg of human neural cortex tissue lysate.

Target description

~65 kDa observed; 73.46 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Physical form

Affinity Purified

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. Quality Level: 100. antibody form: affinity isolated antibody. antibody product type: primary antibodies. clone: polyclonal. purified by: affinity chromatography. species reactivity: human. technique(s): western blot: suitable. NCBI accession no.: NP_055720. UniProt accession no.: O94925. shipped in: ambient. target post-translational modification: unmodified. Gene Information: human ... GLS(2744). Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.