ANTI-GLUCOSE TRANSPORTER-4 RABBIT PAB (C15-1303-222)

General description

Anti-Glucose Transporter-4, rabbit polyclonal, recognizes the ~40-43 kDa GLUT-4. It is validated for use in ELISA, Western blotting, immunoprecipitation, and immunohistochemistry on frozen sections.

Immunoaffinity purified rabbit polyclonal antibody. Recognizes the ~40-43 kDa Glut-4 protein.

Recognizes the ~40-43 kDa GLUT-4 protein.

Immunogen

Mouse

a synthetic peptide corresponding to amino acids near the C-terminus of mouse GLUT-4, conjugated to KLH

Application

ELISA (0.5-1 µg/ml)

Frozen Sections (2-20 µg/ml)

Immunoblotting (1-10 µg/ml; see comments)

Immunoprecipitation (1-10 µg/100 µg tissue membrane)

Packaging

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Standard Handling (A)

Physical form

In PBS, 0.1% BSA, pH 7.5.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Other Notes

Does not cross-react with Hep G2-type transporter or other Glut isoforms. Important: Do not heat samples prior to immunoblotting, as heating will result in protein aggregation. Variables associated with assay conditions will dictate the optimal working dilution.

Mueckler, M. 1994. Eur. J. Biochem. 219, 713.
James, D.E., et al. 1989. Nature 338, 83.
James, D.E., et al. 1989. Proc. Natl. Acad. Sci. USA 86, 8368.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

biological source: rabbit. Quality Level: 100. antibody form: affinity isolated antibody. antibody product type: primary antibodies. clone: polyclonal. form: liquid. contains: ≤. 0.1% sodium azide as preservative. species reactivity: rat, human, mouse. manufacturer/tradename: Calbiochem^®. . storage condition: OK to freeze, avoid repeated freeze/thaw cycles. isotype: IgG. shipped in: wet ice. storage temp.: −. 20°C. target post-translational modification: unmodified. Gene Information: human ... SLC2A4(6517). Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 2. Flash Point(F): Not applicable. Flash Point(C): Not applicable.