Specificity
Recognizes Glut-3 in mouse and rat tissues. C terminal peptide used shows no homology to the human sequence.
Immunogen
Synthetic peptide corresponding to the C-terminus of mouse Glut-3 coupled to KLH. Product sold sold as AG617
Application
Anti-Glucose Transporter GLUT-3 Antibody, C-terminus is an antibody against Glucose Transporter GLUT-3 for use in ELISA & WB.
Western blotting: 1:1,000-1:5,000, using chemiluminescent detection. Detects a 45-48kDa band in membrane preps from rat brain. Slight differences in band sizes in different tissues; reported range is 45-55kDa.
Other detection methods may require a higher concentration of antibody.
ELISA: 1:10,000 - 1:50,000, using 50-100 ng of antigen/well.
Optimal working dilutions must be determined by end user.
Physical form
Liquid rabbit antiserum containing 0.05% sodium azide.
Storage and Stability
Store at -20°C for up to 12 months after date of receipt in undiluted aliquots. Avoid repeated freeze-thaw cycles.
Analysis Note
Control
The control peptide is available for absorbtion studies. Please see catalog number AG617.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
biological source: rabbit. Quality Level: 100. antibody form: serum. antibody product type: primary antibodies. clone: polyclonal. species reactivity: rat, mouse. manufacturer/tradename: Chemicon®. . technique(s): ELISA: suitable, western blot: suitable. NCBI accession no.: NP_035531.3. UniProt accession no.: P32037. shipped in: wet ice. target post-translational modification: unmodified. Gene Information: mouse ... Slc2A3(20527)rat ... Slc2A3(25551). Storage Class Code: 10 - Combustible liquids. WGK: WGK 1.
- UPC:
- 41116010
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- AB1344
