ANTI-GLUD1 ANTIBODY (C15-1317-591)

General description

GLUD1/GDH1 is a mitochondrial glutamate dehydrogenase that converts L-glutamate into alpha keto-glutarate which is an important intermediate in the TCA cycle. GLUD1/GDH1, because it metabolizes glutamate which is also an important neurotransmitter, plays a role in learning and memory reactions mediated by glutamate, as well as certain neurodegenerative disorders associated with altered glutamate metabolism. Mutations in GLUD1/GDH1 are the most common cause of persistent hyperinsulinism hypoglycemia (HHF6). Interestingly GLUD1/GDH1 may also be involved in glutamate toxicity that accompanies aging. It appears that a decline in GLUD1/GDH1 function, even in over expressing mice, leads to buildup of glutamate and subsequent glutamate induced neurotoxicity.

Immunogen

Epitope: Near N-terminal

KLH-conjugated linear peptide corresponding to human Glud1 near the N-terminal.

Application

Immunohistochemistry Analysis: A 1:50-1,000 dilution from a representative lot detected Glud1 in mouse hindbrain, human thalamus, and human cerebral cortex tissue.

Research Category
Neuroscience

Research Sub Category
Developmental Signaling

This Anti-Glud1 Antibody is validated for use in Western Blotting and Immunohistochemistry for the detection of Glud1.

Quality

Evaluated by Western Blotting in human hippocampus tissue lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected Glud1 in 10 µg of human hippocampus tissue lysate.

Target description

~58 kDa observed

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. Quality Level: 100. antibody form: affinity isolated antibody. antibody product type: primary antibodies. clone: polyclonal. purified by: affinity chromatography. species reactivity: human, mouse. species reactivity (predicted by homology): monkey (based on 100% sequence homology), bat (based on 100% sequence homology), baboon (based on 100% sequence homology), bovine (based on 100% sequence homology). technique(s): immunohistochemistry: suitable, western blot: suitable. NCBI accession no.: NP_005262. UniProt accession no.: P00367. shipped in: wet ice. target post-translational modification: unmodified. Gene Information: human ... GLUD1(2746). Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.