General description
L glutamate is the major excitatory neurotransmitter in the central nervous system. Glutamatergic neurotransmission is involved in most aspects of normal brain function and can be perturbed in many neuropathologic conditions. The metabotropic glutamate receptors are a family of G protein coupled receptors that have been divided into 3 groups. Group I includes GRM1 and these receptors have been shown to activate phospholipase C. GRM1 expression has been reported in various regions of the brain and spinal cord. ESTs have been isolated from brain and eye libraries.
Specificity
Predicted to cross-react with Chimpanzee based pm 100% sequence homology.
Recognizes GluR1, Mr 106 kDa.
Immunogen
KLH-conjugated, synthetic peptide corresponding to amino acids 858-868 (..TLPRNSGAGAS..) of human GluR1 (amino acids 840-850 of mature human GluR1)
Application
Anti-GluR1 Antibody, clone C3T detects level of GluR1 & has been published & validated for use in IH, IP & WB.
Immunoprecipitation: 2 μL of a previous lot immunoprecipitated GluR1 from rat brain microsomal preparation.
Immunohistochemistry: A previous lot of this antibody has been shown to detect GluR1 in human, mouse and rat tissues.
Research Category
Neuroscience
Research Sub Category
Neurotransmitters & Receptors
Quality
Routinely evaluated by Western blotting of rat brain microsomal preparation.
Western Blotting Analysis (WB): A 1:5,000-1:10,000 dilution of this lot detected GluR1 in rat brain microsomal preparation (Catalog # 12-144).
Target description
106 kDa
Linkage
Replaces: 05-855
Physical form
Cultured supernantant in 0.05% sodium azide
Storage and Stability
Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Analysis Note
Control
Routinely evaluated by immunoblot on rat brain microsomal preparation (12-144)
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Shipping Information:
Dry Ice Surcharge & Ice Pack Shipments: $40
More Information: https://cenmed.com/shipping-returns
- UPC:
- 51141518
- Condition:
- New
- Availability:
- 3-5 Days
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- MPN:
- 04-855
- Temperature Control Device:
- Yes
