ANTI-GLUR2 ANTIBODY (C005B-239725)

General description

Glutamate receptors (GluRs) can be categorized as ionotropic or metabotropic and subcatergorized by their agonist preferences (NMDA, AMPA or Kainic acid). There are four types of AMPA selective GluR subunits (GluR1, GluR2, GluR3 and GluR4). Tetrameric or pentameric combinations of different subunits contributes to the functional diversity of AMPA receptors. In general, AMPA receptors mediate fast synaptic current at most excitatory synapses, with stoichiometry characterized by subtype composition. Although subunit composition of AMPA receptors varies, they must contain at least one edited GluR2 subunit to be calcium impermeable. Relative calcium permeability in AMPA receptor channels may be significant in pathological neurotoxic damage and long term changes in nervous system responses.

Specificity

This antibody recognizes the cytoplasmic domain of Glutamate Receptor 2.

Immunogen

Epitope: Cytoplasmic domain

KLH-conjugated linear peptide corresponding to the cytoplasmic domain of rat GluR2.

Application

Immunohistochemistry Analysis: A 1:4,000 dilution from a representative lot detected GluR2 in normal human and rat brain tissues.

Research Category
Neuroscience

Research Sub Category
Signaling Neuroscience

This GluR2 antibody is validated for use in WB & IHC for the detection of the GluR2 protein.

Quality

Evaluated by Western Blotting in mouse brain tissue lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected GluR2 in 10 µg of mouse brain tissue lysate.

Target description

~108 kDa observed. Three isoforms at 99 kDa, 99 kDa, and 100 kDa are known to exist due to alternative splicing.
There is no known homology to mouse and rat GluR1, GluR3 and GluR4. There was no homology detected to GluR1 and GluR3 in human, but there is 67% sequence homology to GluR4. An uncharacterized band may be observed at ~75 kDa in some cell lysates.

Linkage

Replaces: AB1768-25UG

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Mouse brain tissue lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. Quality Level: 100. antibody form: affinity isolated antibody. antibody product type: primary antibodies. clone: polyclonal. purified by: affinity chromatography. species reactivity: mouse, rat, human. packaging: antibody small pack of 25 . μ. g. technique(s): immunohistochemistry: suitable (paraffin), western blot: suitable. NCBI accession no.: NP_058957. UniProt accession no.: P19491. shipped in: wet ice. target post-translational modification: unmodified. Gene Information: human ... GRIA2(2891). Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.

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