Specificity
Recognizes Glutamate Receptor 1 [GluR1], phosphoSer831. The antibody recognizes a protein of ~100 kDa corresponding to GluR1 phosphorylated at Ser831 in rat hippocampal homogenate. Immunolabeling of the GluR1 protein is blocked by the immunogen peptide but not by the corresponding non-phosphopeptide. The immunogen sequence is 100% homologous in human and mouse.
Immunogen
Epitope: phosphoSer 831
Synthetic peptide corresponding to amino acids surrounding the phosphoSer831 of rat GluR1.
Application
Detect Glutamate Receptor 1 using this Anti-Glutamate Receptor 1 Antibody, phosphoSer 831 validated for use in WB.
Research Category
Neuroscience
Research Sub Category
Neurotransmitters & Receptors
Western blot and dot blots: 1:500-1:1000. The working dilution should be based on the tissue and the expected phosphorylation state.
Optimal working dilutions must be determined by the end user.
Target description
100 kDa
Linkage
Replaces: 05-823
Physical form
Affinity Purified immunoglobulin. Liquid in 10 mM HEPES, pH 7.5, with 150 mM NaCl, 100 μg/mL BSA and 50% glycerol.
ImmunoAffinity Purified
Storage and Stability
Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Analysis Note
Control
Hippocampal neurons, rat brain tissue lysates or mouse brain tissue lysate
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
biological source: rabbit. Quality Level: 100. antibody form: affinity purified immunoglobulin. antibody product type: primary antibodies. clone: polyclonal. purified by: affinity chromatography. species reactivity: rat. packaging: antibody small pack of 25 . μ. L. manufacturer/tradename: Chemicon®. . technique(s): western blot: suitable. NCBI accession no.: NM_000827.2. UniProt accession no.: P42261. shipped in: dry ice. target post-translational modification: unmodified. Gene Information: human ... GRIA1(2890). Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 2. Flash Point(F): Not applicable. Flash Point(C): Not applicable.- UPC:
- 12352200
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- AB5847
