Anti-Glutathione S-Transferase A1/A2 Antibody (C15-1317-761)

General description

Glutathione S-transferase (GST) A1 (EC:2.5.1.18; UniProt P13745; GST Ya, GST Ya1, GST A1, GST class-alpha member 1) and GST A2 (UniProt P10648; GT41A, GST Ya2, GST A2, GST class-alpha member 2) are encoded by the Gsta1 gene (Gene ID 14857) and the Gsta2 gene (Gene ID 14858), respectively, in murine species. Glutathione S-transferases constitute a family of metabolic isozymes that are involved in detoxification by catalyzing the conjugation of the reduced form of glutathione (GSH) to xenobiotic substrates. The GST family can be subdivided into the cytosolic, mitochondrial, and microsomal (a.k.a. MAPEG) proteins. Mammalian GSTs exist in either as homodimers or heterodimers. The individual monomers were historically characterized by their quaternary structure and size, Yf (Mr 24 500), Yk (25 kDa), Ya1 (25.5 kDa), Ya2 (25.5 kDa), Yn (26.5 kDa), Yb1 (27 kDa), Yb2 (27 kDa) and Yc (28.5 kDa). The “Y” designation refers to the “Y” fraction (as apposed to the “X and Z” fractions) they were found in during purification by Sephadex G75 chromatography. Murine Ya1 and Ya2 are different by only 11 out of their sequences (223 a.a./A1 or 223 a.a./A2). In addition, GST Ya1/Ya2 sequences are higly conserved among murine and rat species (>85% identity), while less sequence homology is found when comparing to the corresponding human proteins. Rat GST YaYa dimer was historically referred to as "Ligandin" or GST L, and old nomenclature also referred to human alpha classe GST A1-1, A1-2 and A2-2 as, respectively, B1B1, B1B2 and B2B2, where B stands for basic.

Specificity

This antiserum cannot distinguish between Glutathione S-transferase A1 and A2, which share more than 95% sequence homology (UniProt P13745 & P10648).

Immunogen

Purified mouse liver Glutathione S-Transferase A1/A2.

Application

Anti-Glutathione S-Transferase A1/A2 Antibody is an antibody against Glutathione S-Transferase A1/A2 for use in Western Blotting.

Research Category
Signaling

Research Sub Category
Signaling Neuroscience

Western Blotting Analysis: A 1:5,000 dilution from a representative lot detected Glutathione S-Transferase A1/A2 in 10 µg of mouse testis and small intestine tissue lysate.
Western Blotting Analysis: A representative lot detected upregulated Glutathione S-transferase A1 & A2 (GSTA1/A2) in liver cytosolic preparations from mice on high-fat diet (HF) when compared with liver samples from mice on regular diet (Meakin, P.J., et al. (2014). Mol. Cell. Biol. 34(17):3305-3320).
Western Blotting Analysis: A representative lot detected anticarcinogen-induced Glutathione S-transferase A1 & A2 (GSTA1/A2) expression in liver cytosolic preparations from C57BL/6 mice received intraperitoneal beta-naphthoflavone (BNF) injection, while such induction was not observed in mouse strain DBA/2 without functional aryl hydrocarbon (Ah) receptor (McLellan, L.I., et al. (1991). Biochem. J. 276(Pt 2):461-469).
Western Blotting Analysis: A representative lot detected antioxidant-induced Glutathione S-transferase A1 & A2 (GSTA1/A2) expression in liver cytosolic preparations from mice fed with dietary 2(3)-t-butyl-4-hydroxyanisole (BHA) supplementation when compared with liver samples from mice without BHA supplementation (McLellan, L.I., and Hayes, J.D. (1989). Biochem. J. 263(2):393-402).

Quality

Evaluated by Western Blotting in mouse stomach tissue lysate.

Western Blotting Analysis: A 1:5,000 dilution of this antibody detected Glutathione S-Transferase A1/A2 in 10 µg of mouse stomach tissue lysate.

Target description

~25 kDa observed

Physical form

Rabbit polyclonal serum with 0.05% sodium azide.

Unpurified

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. Quality Level: 100. antibody form: serum. antibody product type: primary antibodies. clone: polyclonal. species reactivity: mouse. species reactivity (predicted by homology): rat. technique(s): western blot: suitable. NCBI accession no.: NP_032207, NP_032208. UniProt accession no.: P10648, P13745. shipped in: dry ice. target post-translational modification: unmodified. Gene Information: mouse ... Gsta1(14857). Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.