Anti-Glutathione S-transferase A5 (GSTA5) (C15-1317-334)

General description

Glutathione S-transferase A5 (UniProt: Q7RTV2; also known as EC: 2.5.1.18, GST class-alpha member 5, Glutathione S-transferase A5-5) is encoded by the GSTA5 gene (Gene ID: 221357) in human. Glutathione S-transferases are soluble proteins that catalyze the conjugation of reduced glutathione and a variety of electrophiles, including many known carcinogens and mutagens. Glutathione S-transferase A5 is a homodimeric cytoplasmic protein with two glutathione binding sites. The mammalian glutathione S-transferases are active in drug metabolism are classified into the alpha, mu and pi classes. Members of the alpha-class of glutathione S-transferases show substrate specificity for cumene hydroperoxide (CuOOH) and 7-chloro-4-nitrobenz-2-oxa-1, 3-diazole (NBD-C1). They exhibit a number of differences from the characteristic glutathione S-transferase structure. For example, in its domain II, it contains a short 3-residue beta-strand near the C-terminal segment and a longer alpha-7 helix.

Specificity

This rabbit polyclonal antibody detects Glutathione S-transferase A5 in Human Liver. It targets an epitope within 14 amino acids from the internal region.

Immunogen

KLH-conjugated linear peptide corresponding to 14 amino acids from the internal region of human Glutathione S-transferase A5.

Application

Anti-Glutathione S-transferase A5 (GSTA5) Antibody, Cat. No. ABN1347, is a highly specific rabbit polyclonal antibody that detects human Glutathione S-transferase A5 and has been tested in Immunohistochemistry (Paraffin) and Western Blotting.

Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected Glutathione S-transferase A5 (GSTA5) in human liver tissue.

Research Category
Neuroscience

Quality

Evaluated by Western Blotting in human liver tissue lysate.

Western Blotting Analysis: A 1:2,000 dilution of this antibody detected Glutathione S-transferase A5 (GSTA5) in human liver tissue lysate.

Target description

~25 kDa observed; 25.72 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Physical form

Affinity Purified

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. Quality Level: 100. antibody form: affinity isolated antibody. antibody product type: primary antibodies. clone: polyclonal. purified by: affinity chromatography. species reactivity: human. species reactivity (predicted by homology): monkey (based on 100% sequence homology). packaging: antibody small pack of 25 . μ. L. technique(s): immunohistochemistry: suitable (paraffin), western blot: suitable. NCBI accession no.: NM_153699.1. UniProt accession no.: Q7RTV2. target post-translational modification: unmodified. Gene Information: human ... GSTA5(221357). Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.