TrueBlot® rabbit Anti-goat IgG magnetic beads can be used for separation and purification of goat antibodies from serum or goat antibody-labeled components, as well as for immunoassays, immunoprecipitation, and IP Western blots. For antibody purification, rabbit Anti-goat IgG magnetic beads are incubated with the goat antibody solution and then separated by magnets. After the unbound particulates are washed from the beads, the bound antibodies are eluted from the beads using the elution buffer. The beads are then magnetically separated from the eluted solution, which is removed manually. For IP, target specific antibody is incubated with rabbit Anti-goat IgG magnetic beads. The unbound antibody is washed and the sample containing target antigen is added. After unbound particulates are washed from the beads, the purified protein is eluted from the beads using elution buffer. The samples are then resolved by SDS-PAGE and analyzed by Western blotting.
- UPC:
- 12350000
- Condition:
- New
- Availability:
- 3 Days
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 00-1844-50
