Anti-Gqalpha- and G₁₁alpha-Subunits, Internal (283-300) Rabbit pAb (C15-1303-090)

General description

Anti-G_qα- and G₁₁α-Subunits, Internal (283-300), rabbit polyclonal, recognizes the ~42 kDa G_qα- and the ~43 kDa G₁₁α-subunits. Validated for use in WB and neutralization studies.

Protein A purified rabbit polyclonal antibody. Recognizes the ~42 kDa G_qα- and the ~43 kDa G₁₁α-subunit proteins.

Recognizes the ~42 kDa G_qα- and the ~43 kDa G₁₁α-subunits. Does not cross-react with other G-proteins of the non-pertussis toxin-sensitive family.

Immunogen

a synthetic peptide (IMYSHLVDYFPEYDGPQR) corresponding to an internal amino acid sequence shared by human, mouse, and rat G_qα- and G₁₁α-subunits

Application

Immunoblotting (1:1000)

Neutralization Studies (see comments)

Warning

Toxicity: Standard Handling (A)

Other Notes

The antibody recognizes both G_qα and G₁₁α since they both share the internal sequence represented by the immunizing peptide. The antibody may also be useful to block receptor G protein coupling. Does not cross-react with other G proteins of the non-pertussis toxin sensitive family. Variables associated with assay conditions will dictate the proper working dilution.

Wange, R.L., et al. 1991. J. Biol. Chem. 266, 11409.
Pang, I-H., and Sternweis, P.C. 1990. J. Biol. Chem. 265, 18707.
Strathmann, M., et al. 1989. Proc. Natl. Acad. Sci. USA86, 7407.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

biological source: rabbit. Quality Level: 100. antibody form: purified antibody. antibody product type: primary antibodies. clone: polyclonal. form: liquid. does not contain: preservative. species reactivity: human, mouse, rat. manufacturer/tradename: Calbiochem^®. . storage condition: OK to freeze, avoid repeated freeze/thaw cycles. isotype: IgG. shipped in: wet ice. storage temp.: −. 70°C. target post-translational modification: unmodified. Gene Information: human ... GNAI1(2770)
mouse ... Gnai1(14677)
rat ... Gnai1(25686). Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.