Anti-Green Fluorescent Protein Antibody (C15-1316-012)

General description

The Green Fluorescent Protein (GFP) from the jellyfish Aequorea victoria is used as a fluorescent indicator for monitoring gene expression in a variety of cellular systems, including living organisms and fixed tissues. Unlike other bioluminescent reporters, GFP fluoresces in the absence of substrates, cofactors, or other intrinsic or extrinsic proteins. Purified GFP is a 27 kDa monomer consisting of 238 amino acids and emits green light (emission maximum at 509 nm) when excited with blue or UV light.

Specificity

AB3080P is made against highly-purified native GFP from Aequorea victoria. It is reactive with GFP from both native and recombinant sources. While other commercial antibodies to GFP typically demonstrate strong reactivity with E. coli, the AB3080P GFP antibody has been affinity purified, followed by extensive adsorption against whole E. coli BL21 strain cells, giving them minimal cross-reactivity with E. coli.

Reactivity with other species has not been confirmed.

Immunogen

Highly purified native GFP from Aequorea victoria.

Application

Anti-Green Fluorescent Protein Antibody detects level of Green Fluorescent Protein & has been published & validated for use in ELISA, IC & WB.

Research Category
Epitope Tags & General Use

Research Sub Category
Epitope Tags

Western Blot:
A 1:500-1:1000 dilution of a previous lot was used using colorimetric detection (Peroxidase/TMB), with higher dilution possible using more sensitive detection methods.

ELISA:
A 1:800-1:1,000 dilution of a previous lot was used in ELISA.

Note: While AB3080 has been adsorbed against E. coli proteins, most secondary antibodies have not. To reduce the likelihood of E. coli reactivity from a secondary antibody, we suggest the use of Millipore′s highly purified secondary antibodies in indirect detection systems.

Immunocytochemistry:

Optimal working dilutions and protocols must be determined by end user.

Quality

Routinely evaluated by immunocytochemistry on RENCX cells.

Immunocytochemistry Analysis: Confocal fluorescent analysis of RENCX constitutively expressed GFP using this lot′s anti-GFP Rabbit pAB (Red). Green indicates the expressed GFP.

Target description

Varies (GFP is 27 kDa)

Physical form

Affinity purified rabbit immunoglobulin

Affinity purified rabbit polyclonal IgG in 10 mM Tris buffer.

Storage and Stability

Stable for 1 year at -20°C in undiluted aliquots from date of receipt.

Handling Recommendations:
Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C.

Analysis Note

Control
Cells transfected with GFP-tagged fusion vector

RENCX cells

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. Quality Level: 100. antibody form: affinity purified immunoglobulin. antibody product type: primary antibodies. clone: polyclonal. purified by: affinity chromatography. species reactivity: vertebrates (most common). species reactivity (predicted by homology): rat. manufacturer/tradename: Chemicon^®. . technique(s): ELISA: suitable, immunocytochemistry: suitable, western blot: suitable. UniProt accession no.: P42212. shipped in: dry ice. target post-translational modification: unmodified. Storage Class Code: 12 - Non Combustible Liquids. WGK: nwg. Flash Point(F): Not applicable. Flash Point(C): Not applicable.