Anti-GRK2 antibody, Mouse monoclonal

General description

G protein-coupled receptor kinases (GRKs) protein family has 6 members, ranging from GRK1-GRK6.

Monoclonal Anti- GRK2/ARK1 (mouse IgG2b isotype) is derived from the hybridoma 3A10 produced by the fusion of mouse myeloma cells (SP2/0-Ag14 cells) and splenocytes from BALB/c mice immunized with recombinant bovine GRK2/ARK. G protein-coupled receptor kinases (GRKs) are important regulators of G protein-coupled receptors (GPCRs). GRK2, one of six members of this family that have been identified to date, is ubiquitously expressed in mammals. G-protein-coupled receptor kinase 2 (GRK2), which is officially known as adrenergic, , receptor kinase 1 (ADRBK1), gene codes for a 689 amino acid protein, that is of 79 kDa. This gene has 12 catalytic region subdomains. GRK2 is expressed in the vascular endothelium, arterial smooth muscle, myocardium, kidney and in the renal proximal tubule. It is mapped to human chromosome 11q13.

Specificity

Monoclonal Anti-GRK2 antibody is specific for human, rat, and bovine GRK2/bARK1 (approx. 65 kDa). The antibody epitope is present within a highly conserved region of GRK2, ranging from residues 500-531. The product does not bind to other GRKs.

Immunogen

recombinant bovine GRK2.

Application

Anti-GRK2 antibody, Mouse monoclonal has been used in:

• immunoblotting
• immunoprecipitation
• immunofluorescence staining

Monoclonal Anti-GRK2 antibody is suitable for use in western blot (2-4 μg/mL using total cell extract from rat osteosarcoma), microarray and immunoprecipitation.

Biochem/physiol Actions

G-protein-coupled receptor kinase 2 (GRK2) controls several intracellular mechanisms. It helps to maintain the structure and activities of the heart. Hence it participates in several cardiovascular diseases. GRK2 also modulates ion and fluid transport and eventually, blood pressure. It helps to enhance the production of mitochondrial superoxide and decreases oxygen utilization and ATP synthesis. This protein serves as a major oncomodulator. GRK2 regulates the level and rate of desensitization in GPCRs, and is also involved in cardiovascular pathologies and chemokine receptor modulations.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.