Anti-Gsalpha-Subunit, C-Terminal (385-394) Rabbit pAb (C15-1303-088)

General description

Anti-G_sα-Subunit, C-Terminal (385-394), rabbit polyclonal, recognizes both large and small forms of G_sα-subunit. It is validated for use in Western blotting.

Protein A purified rabbit polyclonal antibody. Recognizes the ~40-45 kDa G_sα subunit protein.

Recognizes both large and small forms of G_sα-subunit. Does not cross-react with G_iα-1-, G_iα-2-, G_iα-3-, or G_oα-subunits.

Immunogen

a synthetic peptide (RMHLRQYELL) (Cat. No. 371782) corresponding to amino acids at the C-terminus of mammalian G_sα subunit, conjugated to KLH

Application

Immunoblotting (1:1000)

Warning

Toxicity: Standard Handling (A)

Physical form

In 140 mM NaCl, 100 mM potassium phosphate, pH 7.5.

Reconstitution

Following initial thaw, aliquot and freeze (-70°C).

Analysis Note

Positive Control
G_sα-Subunit, His•Tag, Rat Brain, Recombinant, E. coli (Cat. No. 371765) or G_sα-Subunit, Recombinant, E. coli, Immunoblot Standard (Cat. No. 371764)

Other Notes

Does not cross-react with G_iα-1, G_iα-2, G_iα-3, or G_oα. The specificity and cross-reactivity were confirmed with lysates from separate cultures of bacteria transfected with the genes for G_sα, G_iα-1, G_Iα-2, G_iα-3, and G_oα. Variables associated with assay conditions will dictate the proper working dilution.

Kumar, R., et al. 1994. J. Mol. Cell. Cardiol.26, 1537.
Raymond, J.R., et al. 1993. Biochemistry32, 11064.
Mumby, S.M., and Gilman, A.G. 1991. Methods Enzymol.195, 215.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

biological source: rabbit. Quality Level: 100. antibody form: purified antibody. antibody product type: primary antibodies. clone: polyclonal. form: liquid. does not contain: preservative. species reactivity (predicted by homology): mammals. manufacturer/tradename: Calbiochem^®. . storage condition: OK to freeze, avoid repeated freeze/thaw cycles. isotype: IgG. shipped in: wet ice. storage temp.: −. 70°C. target post-translational modification: unmodified. Gene Information: human ... GNAI1(2770). Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.