Anti-GSDMD (126-138) antibody produced in rabbit (C15-1425-768)

General description

Gasdermin D (GSDMD) is also known as DFNA5L or gasdermin domain-containing 1 (GSDMDC1), belongs to the gasdermin protein family of epithelial proliferation regulators. GSDMD is expressed in upper gastrointestinal epithelium differentiating cells.

Immunogen

synthetic peptide corresponding to amino acids 126-138 of human GSDMD

Application

Anti-GSDMD (126-138) antibody produced in rabbit has been used in:

• immunoblotting
• CASP4/11 assay
• cytokine enzyme?linked immunosorbent assay

Anti-GSDMD antibody produced in rabbit is suitable for western blot analysis at a working dilution of 1:500-1:2000.
Yale Center for High Throughput Cell Biology IF-tested antibodies. Each antibody is tested by immunofluorescence against HUVEC cells using the Yale HTCB IF protocol. To learn more about us and Yale Center for High Throughput Cell Biology partnership, visit sigma.com/htcb-if.

Biochem/physiol Actions

GSDMD (gasdermin D) have been suggested to function in the regulation of epithelial apoptosis. GSDMD is expressed in differentiating cells. It may act as a tumor suppressor.

The absence of Gasdermin D (GSDMD), completely blocked LPS electroporation-triggered pyroptosis in studies performed on GSDMD siRNA knockdown or GSDMD knockout (both human HeLa cells and iBMDM mouse cells). It was also reported that caspase-4/11 specifically cleaves GSDMD after Asp275, results in gasdermin-N domain that bears intrinsic pyroptosis inducing activity. Furthermore, GSDMD-mediated pyroptosis participates in mature interleukin 1β (IL-1β) release without distressing its maturation.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.