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ANTI-HA-PEROXIDASE HIGH AFFINITY (3F10)

Catalog No.
C15-1297-745
Manufacturer No.
12013819001
Manufacturer Name
Sigma-Aldrich
Quantity
25
Unit of Measure
UN
Price: $661.44
List Price: $734.93

Anti-HA-Peroxidase, High Affinity is a monoclonal antibody to the HA-peptide (clone 3F10), conjugated to peroxidase. Specificity Anti-HA-Peroxidase, High Affinity (3F10) recognizes the 9-amino acid sequence YPYDVPDYA, derived from the human

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General description

Anti-HA-Peroxidase, High Affinity is a monoclonal antibody to the HA-peptide (clone 3F10), conjugated to peroxidase.

Specificity

Anti-HA-Peroxidase, High Affinity (3F10) recognizes the 9-amino acid sequence YPYDVPDYA, derived from the human influenza hemagglutinin (HA) protein.
This epitope is also recognized in fusion proteins regardless of its position (N-terminal, C-terminal or internal).

Immunogen

The epitope consists of amino acids 98-106 from the human influenza virus hemagglutinin protein.

Application

  • Use Anti-HA-Peroxidase, High Affinity for the detection of HA-tagged recombinant proteins using: ELISA
  • Western blot
Since Anti-HA High Affinity is a rat monoclonal, it is possible to use it in conjunction with murine monoclonals for double labeling.

Preparation Note

Stabilizers: proteinaceous stabilizers
Working concentration: The working concentration of conjugate depends on application and substrate.
The following concentrations should be taken as a guideline:

  • Dot blot: 50 mU/ml
  • ELISA: 25 mU/ml
  • Western blot: 50 mU/ml

Reconstitution

Add 1.0 ml double-distilled water for a final concentration of 25 U/mL.
Rehydrate for 10 min prior to use.

Other Notes

For life science research only. Not for use in diagnostic procedures.

UPC:
51473503
Condition:
New
Weight:
1.00 Ounces
HazmatClass:
No
WeightUOM:
LB
MPN:
12013819001


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