Anti-hABH1 antibody, Mouse monoclonal (C15-1315-446)

General description

Anti-hABH1 antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the hABH1-151 hybridoma produced by the fusion of mouse myeloma cells (NS1 cells) and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to a fragment of human hABH1, conjugated to keyhole limpet hemocyanin (KLH).

Human AlkB homologue 1 (hABH1) is one of the eight homologs of E. coli AlkB. Homology modeling studies have analyzed the role of hABH1 gene in DNA repair after alkaylation-induced damage.

Specificity

Monoclonal Anti-hABH1 (human AlkB homologue) recognizes human and monkey ABH1. It does not recognize hABH2 or hABH3. The antibody epitope resides within amino acids 19-34 of human hABH1.

Application

Anti-hABH1 antibody, Mouse monoclonal has been used in

• chromatin immunoprecipitation(ChIP)
• immunohistochemistry
• enzyme-linked immunosorbent assay (ELISA)
• immunoblotting
• immunoprecipitation

Biochem/physiol Actions

The human homolog, AlkB homolog 1, histone H2A dioxygenase (Alkbh1) is linked with several enzymatic actions, such as, DNA, mRNA or tRNA hydroxylation, cleavage at abasic (AP) site in DNA and demethylation of histones. It plays a key role in epigenetic gene silencing, as it is known to exhibit m6A demethylation activity . ALKBH1 participates in normal cell proliferation.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: mouse. Quality Level: 200. conjugate: unconjugated. antibody form: purified immunoglobulin. antibody product type: primary antibodies. clone: hABH1-151, monoclonal. form: buffered aqueous solution. mol wt: antigen ~45 . kDa. species reactivity: human, monkey. technique(s): immunohistochemistry: suitable, immunoprecipitation (IP): suitable, indirect ELISA: suitable, microarray: suitable, western blot: 1-2 . μ. g/mL using total cell extract of 293T cells. isotype: IgG1. UniProt accession no.: Q13686. shipped in: dry ice. storage temp.: −. 20°C. target post-translational modification: unmodified. Gene Information: human ... ALKBH1(8846). Storage Class Code: 10 - Combustible liquids. WGK: WGK 2. Flash Point(F): Not applicable. Flash Point(C): Not applicable.