Anti-HD-polyLeu-Ct (C15-1317-419)

General description

Huntington disease (HD) is a progressive neurodegenerative disorder is caused by a CAG,CTG expansion in the HTT gene that results in the production of a mutant huntingtin protein (HTT) with polymeric expansions that accumulate in human brain with HD. PolyLeu is an antisense repeat-associated non-ATG (RAN) translation protein that accumulate in various brain regions with toxic effects leading to microglial activation and neuronal loss. HD-RAN polyLeu toxicity is shown to be comparable to that of polyGln. HD-RAN proteins are abundant in regions of the brain that are most affected by HD and these regions display pathologic features of HD, such as caspase-3 activity and microglial activation. HD-Ran protein accumulation and aggregation in HD brains are shown to be length dependent.

Specificity

This rabbit polyclonal antibody specifically detects RAN proteins with PolyLeu-HD.

Immunogen

A synthetic peptide corresponding to the C-terminal region of the predicted polyLeu frames of HD in the CTG direction (HD-Leu-Ct).

Epitope: unknown

Application

Anti-HD-polyLeu-Ct, Cat. No. ABN1670, is a highly specific rabbit polyclonal antibody that targets HD-polyLeu-Ct and has been tested for use in Immunocytochemistry, Immunohistochemistry, and Western Blotting.

Research Category
Neuroscience

Western Blotting Analysis: A 1:2,000 dilution from a representative lot detected HD-polyLeu-Ct in HEK293T cells transfected with HD-polyLeu-Ct.

Immunocytochemistry Analysis: A representative lot detected HD-polyLeu-Ct in Immunocytochemistry applications (Banez-Coronel, M., et. al. (2015). Neuron. 88(4):667-77).

Western Blotting Analysis: A representative lot detected HD-polyLeu-Ct in Western Blotting applications (Banez-Coronel, M., et. al. (2015). Neuron. 88(4):667-77).

Immunohistochemistry Analysis: A representative lot detected HD-polyLeu-Ct in Immunohistochemistry applications (Banez-Coronel, M., et. al. (2015). Neuron. 88(4):667-77).

Quality

Evaluated by Western Blotting in HEK293T cells transfected with HD-polyLeu-Ct.

Western Blotting Analysis (WB): A 1:2,000 dilution of this antibody detected PolyLeu in HEK293T cells transfected with HD-polyLeu-Ct, but not in control cells.

Target description

~21 kDa observed. Uncharacterized bands may be observed in some lysate(s).

Physical form

Rabbit polyclonal antiserum with 0.05% sodium azide.

Unpurified

Storage and Stability

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. antibody form: serum. antibody product type: primary antibodies. clone: polyclonal. species reactivity: human. packaging: antibody small pack of 25 . μ. L. technique(s): immunocytochemistry: suitable, immunohistochemistry: suitable, western blot: suitable. isotype: IgG. target post-translational modification: unmodified.