Specificity
MAB88079 recognizes a protein of 70 kDa, identified as Heat Shock Factor 2 (HSF2). The synthesis of heat shock proteins is dependent on transcriptional and post-transcriptional control in eukaryotic cells. The regulation of heat shock gene transcription is mediated by the transcriptional activator, Heat Shock Factor (HSF), which binds to heat shock response elements (HSEs). At least three HSFs have been isolated from the human, mouse, chicken, and tomato genomes and an additional factor, HSF4 has been identified in humans. HSFs purified from yeast, Drosphila, and human have different molecular weights and do not cross-react immunologically. These factors are activated by distinct stimuli. HSF1 responds to cellular stress signals such as heat, heavy metals, and oxidative reagents whereas, the HSF2 is activated during differentiation. some laboratories have reported that the constitutive, non-DNA binding, monomeric form of inactive HSF1 is located in the cytoplasm. Upon activation, HSF1 is trimerized and is localized in nucleus where it binds DNA. the phosphorylation of HSF1 may be necessary for maximal transcription of heat shock genes.
Immunogen
Full length recombinant mouse HSF2 proteins (aa 1-517)
Application
Anti-Heat Shock Factor II Antibody, clone 3E2 is an antibody against Heat Shock Factor II for use in ELISA, IF, IP & WB.
Immunofluorescence Immunohistology (predominantly cytoplasmic with some nuclear). Immunoprecipitation: 2 μg/mg protein lysate. Western blot. 1:200-1:400 ELISA
Optimal working dilutions must be determined by end user.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Transcription Factors
Physical form
Format: Purified
Purified immunoglobulin. Liquid containing 10 mM PBS, pH 7.4, with 0.2% BSA and 15 mM sodium azide.
Storage and Stability
Maintain refrigerated at 2-8°C in undiluted aliquots for up to 12 months.
Analysis Note
Control
POSITIVE CONTROL:
NIH-3T3 cells
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Shipping Information:
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- UPC:
- 12352200
- Condition:
- New
- Availability:
- 3-5 Days
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- MPN:
- MAB88079
- Temperature Control Device:
- Yes
