Specificity
Reacts with both the constitutive (HSP73) and inducible (HSP72) forms of HSP70.
POSITIVE CONTROL:
HeLa cells, BT474 cells or breast carcinoma.
Immunogen
HSP70 from HeLa cells.
Application
Immunoblotting: 0.5-1 μg/mL
Immunohistochemistry (frozen and formalin/paraffin): 2-4 μg/mL for 2 hours at room temperature. Staining of formalin fixed tissue sections requires boiling the tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 minutes followed by cooling at room temperature for 20 minutes.
Immunoprecpitation: 2 μg/mg protein lysate (use Protein G)
Flow cytometry
Optimal working dilutions must be determined by end user.
This Anti-Heat Shock Protein 70 Antibody, clone W27 is validated for use in IP, WB, IH(P) for the detection of Heat Shock Protein 70.
Physical form
Format: Purified
Analysis Note
Control
POSITIVE CONTROL: BT474, MAD109, RenCa, SK-NEP-1, U937 cells or breast carcinoma.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Shipping Information:
Dry Ice Surcharge & Ice Pack Shipments: $40
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- UPC:
- 51201651
- Condition:
- New
- Availability:
- 3-5 Days
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- MPN:
- MAB3516
- Temperature Control Device:
- Yes
