General description
Hes1 is a transcriptional repressor of genes that require a bHLH protein for their transcription. It may act as a negative regulator of myogenesis by inhibiting the functions of MYOD1 and ASH1. Hes1 is expressed in developing neuroectodermal and endodermal endocrine tissues, and Hes1 deficient embryos show severe defects in neuronal development, as well as pancreatic hypoplasia.
Specificity
Reactivity with other species has not been confirmed.
Recognizes HES-1 (Hairy family), a Helix-loop-helix class of transcription factor.
Immunogen
Synthetic peptide.
Application
Research Category
Neuroscience
Research Sub Category
Developmental Neuroscience
Neuronal & Glial Markers
This Anti-HES-1 Antibody is validated for use in IH, WB for the detection of HES-1.
Western Blot:
1:200-1:1,000 dilution using ECL.
Immunohistochemistry:
1:200-1:1,000 dilution of a previous lot worked.
Optimal working dilutions must be determined by the end user.
Target description
29 kDa
Physical form
ImmunoAffinity Purified
Purified rabbit polyclonal in buffer containing a solution of 50% saturated ammonium sulfate and PBS containing no preservatives.
Storage and Stability
Stable for 1 year at -80ºC from date of receipt.
Analysis Note
Control
Human neural stem cells, Raji cell lysate.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
biological source: rabbit. Quality Level: 100. antibody form: affinity isolated antibody. antibody product type: primary antibodies. clone: polyclonal. purified by: affinity chromatography. species reactivity: rodent, human, mouse. manufacturer/tradename: Chemicon®. . technique(s): immunohistochemistry: suitable, western blot: suitable. NCBI accession no.: NM_005524.2. UniProt accession no.: Q14469. shipped in: dry ice. target post-translational modification: unmodified. Gene Information: human ... HES1(3280)mouse ... Hes1(15205). Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 2. Flash Point(F): Not applicable. Flash Point(C): Not applicable.
- UPC:
- 12352203
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- AB5702
