General description
Histones are highly conserved proteins that serve as the structural scaffold for the organization of nuclear DNA into chromatin. The four core histones, H2A, H2B, H3, and H4, assemble into an octamer (2 molecules of each). Subsequently, 146 base pairs of DNA are wrapped around the octamer, forming a nucleosome. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into 30nm chromatin fibers and higher order structures.
Specificity
This antibody recognizes Histone H1°.
Immunogen
Recombinant protein corresponding to Ox liver Histone H1°
Application
Anti-Histone H1° Antibody, clone 34 is a highly specific mouse monoclonal antibody, that targets Histone H1 & has been tested in western blotting, ICC, IHC & Flow Cytometry.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Histones
Western Blotting Analysis: A representative lot from an independent laboratory detected Histone H1° in Xenopus embryo tissue lysate (Seigneurin, D., et al. (1995). Int J Dev Biol. 39(4):597-603.; Fu, G., et al. (2003). Biol Reprod. 68(5):1569-1576.).
Immunocytochemistry Analyis: A representative lot from an independent laboratory detected Histone H1° in Xenopus unfertilized eggs and early embryos (Fu, G., et al. (2003). Biol Reprod. 68(5):1569-1576.; Adenot, P. G., et al. (2000). J Cell Sci. 113(Pt 16):2897-2907.).
Immunohistochemistry Analysis: A representative lot from an independent laboratory detected Histone H1° in Xenopus embryo tissues (Grunwald, D., et al. (1995). Exp Cell Res. 218(2):586-595.).
Flow Cytometry Analyisis: A representative lot from an independent laboratory detected Histone H1° in FC (Grunwald, D., et al. (1999). Methods Mol Biol. 119:443-454.).
Quality
Evaluated by Western Blotting in Jurkat cell lysate.
Western Blotting Analysis: 1 µg/mL of this antibody detected Histone H1° in 10 µg of Jurkat cell lysate.
Target description
~30 kDa observed. Uncharacterized band(s) may be observed in some cell lysates.
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
Jurkat cell lysate
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Shipping Information:
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- UPC:
- 41181931
- Condition:
- New
- Availability:
- 3-5 Days
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- MPN:
- MABE446
- Temperature Control Device:
- Yes
