ANTI-HISTONE H1E FRAMESHIFT CLONE 1D8 ZOOMAB RABBIT MONOCLONAL (C15-1670-738)

General description

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Specificity

Clone 1D8 a ZooMAb^® Mouse recombinant monoclonal antibody that specifically detects Histone H1 with H1E frameshift mutation.

Immunogen

KLH-conjugated linear peptide corresponding to a modified 12 amino acids sequence from the human Histone H1E.

Application

Quality Control Testing

Evaluated by Western Blotting in Human oligodendroglioma cells expressing wild-type, heterozygous fs, or homozygous fs H1E.

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected Histone H1E frameshift in Human oligodendroglioma cells expressing heterozygous fs (B39), or homozygous fs (A41) H1E, but not the wild-type H1E.

Tested applications

Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected Histone H1E frameshift in Oligodendroglioma cells transfected with Histone H1E frameshift transgene (Data courtesy of Dr. Alexey Soshnev and Dr C. David Allis, Rockefeller University).

Chromatin Immunoprecipitation Analysis (ChIP): A representative lot detected Histone H1E frameshift in ChIP analysis of Oligodendroglioma cells transfected with Histone H1E frameshift or wildtype H1E transgenes (Data courtesy of Dr. Alexey Soshnev and Dr. C. David Allis, Rockefeller University).

Immunohistochemistry Applications: A representative lot detected H1E frameshift, clone 1D8 ZooMAbZooMAb^® Rabbit Monoclonal in Immunohistochemistry applications Wildtype and Histone H1E Frameshift transgenic mouse brain (Data courtesy of Dr. Alexey Soshnev and Dr. C. David Allis, Rockefeller University).

Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected Histone H1E frameshift in Human blood, mouse embryonic stem cells (ESCs), and human oligodendroglioma cells expressing H1E frameshift (Data courtesy of Dr. Alexey Soshnev and Dr. C. David Allis, Rockefeller University).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Target description

Histone 1, H1e (UniProt: A3R0T8; also known as Histone H1.4, HIST1H1E) is encoded by the HIST1H1E (also known as hCG_16457421) gene (Gene ID: 3008) in human. Histones have been classified under five major classes. The core histones H2A, H2B, H3, and H4 constitute the protein components of nucleosome core particles and histone H1 is a linker histone that plays a role in chromatin folding. Depletion of histone H1 can cause dramatic changes in chromatin structure, global nucleosome spacing, and reduced chromatin compaction. Pathogenic mutations in HIST1H1E gene result in the formation of histone H1 that is less effective in neutralizing negatively charged linker DNA and display reduced DNA binding and protein-protein interactions due to truncation in key residues. These mutations are reported to result in a shift in reading frame in the C-terminal region and produce truncated proteins. Frameshift mutations in HIST1H1E have been linked to Rahman syndrome that is manifested in overgrowth with intellectual disabilities (OGID) in early infancy. Subjects with Rahman syndrome display a global tendency in reduction of methylation state. This ZooMAbZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals (Ref.: Andrea Ciolfi, et al. (2020). Clinical Epigenetics 12; Article 7; Tatton-Brown , K., et al. (2017). Am. J. Hum. Genet. 100(5); 725-736).

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitution

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.