ANTI-HISTONE H3.3 ANTIBODY

General description

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine. Histone variant H3.3 is typically enriched in active chromatin.

Specificity

Broad species cross-reactivity expected based on 100% sequence homology.

Immunogen

KLH-conjugated linear peptide corresponding to human Histone H3.3.

Application

Immunocytochemistry Analysis: A representative lot was used by an independent laboratory to detect Histone H3.3 in HeLa cells.
Image courtesy of Simon Elsässer of David Allis′ Laboratory, Rockefeller University, New York.

Chromatin Immunoprecipitation Analysis: A representative lot was used by an independent laboratory in ChIP.
Image courtesy of Simon Elsässer of David Allis′ Laboratory, Rockefeller University, New York.

Dot Blot Analysis: A representative lot was used by an independent laboratory in DB.
Reported by Simon Elsässer, Laboratory of Dr. David Allis, Rockefeller University, New York.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Histones

Use Anti-Histone H3.3 Antibody (Rabbit Polyclonal Antibody) validated in WB, ICC, DB, ChIP to detect Histone H3.3 also known as Histone H3.3.

Quality

Evaluated by Western Blot in HeLa acid extract.

Western Blot Analysis: 1 µg/mL of this antibody detected Histone H3.3 on 10 µg of HeLa acid extract.

Target description

~17 kDa observed

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
HeLa acid extract

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.