General description
NO66 is a lysine-specific demethylase that removes methyl groups from histone H3 at lysine 4 and lysine 36. This enzyme has been shown to more readily demethylate trimethylated H3 ′Lys-4′ (H3K4me3) and monomethylated H3 Lys4 (H3K4me1) consistent with its role in modulating the histone code. This protein is a highly conserved, constitutive nucleolar component that shows a dual localization pattern with strong enrichment in the granular part of nucleoli and in distinct nucleoplasmic entities.
Specificity
This antibody recognizes Histone lysine demethylase NO66 near the N-terminus.
Immunogen
Epitope: Near N-terminus
KLH-conjugated linear peptide corresponding to human Histone lysine demethylase NO66 near the N-terminus.
Application
Detect NO66 using this rabbit polyclonal antibody, Anti-Histone lysine demethylase NO66 Antibody validated for use in western blotting.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Chromatin Biology
Quality
Evaluated by Western Blotting in A431 cell lysate.
Western Blotting Analysis: 2.0 µg/mL of this antibody detected Histone lysine demethylase NO66 in 10 µg of A431 cell lysate.
Target description
~66 kDa observed
Physical form
Antigen Affinity Purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
A431 cell lysate
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
biological source: rabbit. Quality Level: 100. antibody form: affinity isolated antibody. antibody product type: primary antibodies. clone: polyclonal. purified by: affinity chromatography. species reactivity: human. technique(s): western blot: suitable. NCBI accession no.: NP_078920. UniProt accession no.: Q9H6W3. shipped in: wet ice. target post-translational modification: unmodified. Gene Information: human ... RIOX1(79697). Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.- UPC:
- 51312702
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- ABE492