ANTI-HISTONE MACRO H2A.1 CLONE 1M7 ZOOMAB« RABBIT MONOCLONAL (C15-1669-718)

General description

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Specificity

Clone 1M7 is a ZooMAb^® rabbit recombinant monoclonal antibody that specifically detects Histone Macro H2A.1. It targets an epitope within 17 amino acids from the C-terminal half.

Immunogen

KLH-conjugated linear peptide corresponding to 17 amino acids from the C-terminal half in isoform 2 of human Histone Macro H2A.1.

Application

Quality Control Testing

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected Histone Macro H2A.1 in HeLa cell lysate.

Tested Applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Histone Macro H2A.1 in Neuro-2a cell lysate.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Histone Macro H2A.1 in Human cerebral cortex tissue sections.

Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected Histone Macro H2A.1 in HeLa cells.

Affinity Binding Assay: A representative lot of this antibody bound Histone Macro H2A1 peptide with a KD of 1.7 x 10-7 in an affinity binding assay.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Target description

Core histone macro-H2A.1 (UniProt: O75367; also known as Histone macroH2A1, mH2A1, Histone H2A.y, H2A/y, Medulloblastoma antigen MU-MB-50.205) is encoded by the MACROH2A1 (also known as H2AFY) gene (Gene ID: 9555) in human. Histone Macro H2A.1 (mH2A1) is a variant of histone H2A that replaces conventional H2A in a subset of nucleosomes where it represses transcription. Three isoforms of Histone Macro H2A.1 have been described that are produced by alternative splicing.  Isoform 1 specifically binds poly-ADP-ribose and O-acetyl-ADP-ribose and plays a KEY^® role in NAD+ metabolism. It is able to bind to the ends of poly-ADP-ribose chains created by PARP1 and cap them. This prevents PARP1 from further addition of ADP-ribose and thus limits the consumption of NuCLEAR^™ NAD+, allowing the cell to maintain proper NAD+ levels in both the nucleus and the mitochondria to promote proper mitochondrial respiration. Isoform 2 does not bind poly-ADP-ribose. The macro domain of mH2A1 that resides in residues 184-367 is responsible for poly-ADP-ribose binding. mH2A1 is reported to inhibit the binding of transcription factors, including NF-kB, and interfere with the activity of remodeling SWI/SNF complexes. It also inhibits histone acetylation by EP300 and can recruit class I histone deacetylases that induces a hypoacetylated state of chromatin. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, Affinity^™, reproducibility, and stability over conventional monoclonals. (Ref.: Hurtado-Bages, S., et al. (2018). Front. Genet. 9; 417; Doyen, C-M., et al. (2006). Mol. Cell Biol. 26(3); 1156-1164; Angelov, D., et al. (2003). Mol. Cell. 11(4); 1033-1041).

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Reconstitution

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

Affinity is a trademark of Mine Safety Appliances Co.

KEY is a registered trademark of Hanna Instruments

NuCLEAR is a trademark of Sigma-Aldrich Co. LLC

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.