General description
The previously assigned protein identifier P03989 has been merged into P01889. Full details can be found on the UniProt database.
Specificity
This antibody binds strongly to the HLA-B27 antigen. It also binds less strongly to the HLA-B7 antigen. Affinity studies by Scatchard analysis showed that the antibody has a higher affinity for HLA-B27 (9.7x108M-1) than for HLA-B7
(9.5x107M-1).
Cell reactivity:
Flow cytometric analysis of lymphocyte staining for HLA-B27 gave the following results:
Subjects Fluorescence Intensity
Heterozygous HLA-B27+ Strong
Homozygous HLA-B7+ Strong
Heterozygous HLA-B7+ Faint
Non B27, Non B7 Negative
Application
This Anti-HLA-B27 Antibody, clone HLA.ABC.m3 is validated for use in FC, IC for the detection of HLA-B27.
This antibody is useful in the investigation of HLA-B27 in diseases such as ankylosing spondylitis and anterior uveitis.
SUGGESTED USAGE DILUTION
1. Flow cytometry and indirect immunofluorescence 1:60 to 1:120
Dilute with isotonic buffer. Use 50 μl of diluted antibody per 1 x 10E6 peripheral blood mononuclear cells (PBMC) in 100 μl buffer.
2. Cytotoxicity testing 1:25.
Physical form
Format: Purified
The antibody is supplied in phosphate-buffered saline, pH 7.4, containing 0.2% bovine serum albumin and 0.1% sodium azide. The characteristics of each lot are tested by flow cytometry.
Storage and Stability
Store at 2 to 8°C. For prolonged periods, store below -20°C in undiluted aliquots. AVOID REPEATED FREEZE/THAW CYCLES.
WARNING: The monoclonal reagent solution contains 0.1% sodium azide as a preservative. Due to potential hazards arising from the build up of this material in pipes, spent reagent should be disposed of with liberal volumes of water.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Shipping Information:
Dry Ice Surcharge & Ice Pack Shipments: $40
More Information: https://cenmed.com/shipping-returns
- UPC:
- 51201516
- Condition:
- New
- Availability:
- 3-5 Days
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- MPN:
- MAB1285
- Temperature Control Device:
- Yes
