Cell Line Origin
Mouse x Mouse hybridoma
Cell Line Description
The hybridoma line R1 has been obtained by fusion of the myeloma P3x63Ag8.653 and spleen cells from (C57Bl/6 x BALB/c)F1 female mice 3 days after a single i.p. injection of lymphoblastoid cells (LCL). The cell line Rebma, which is homozygous for HLA antigens A2, Bw60, Cw3 and DRw6 was used as LCL source. The antibody produced has been analysed in the International Histocompatibility Workshops 10 (1987; 10WS3086)and 11(11WS0936). R1 has been eradicated from mycoplasma using Cibrobay and is also known as R1A1.
Immunogen
B cell line Rebma HLA A2, Bw60, Cw3,DRw6
Application
Immunofluorescence, Cytometry, Immunoperoxidase, Immunoprecipitation
Culture Medium
RPMI 1640 + 2mM glutamine + 10% FBS
Subculture Routine
Maintain cultures between 3-9 x 100,000 cells/ml; 5% CO2;37°C
Other Notes
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biological source: Mouse x Mouse Hybridoma. ImmunoDon: (BALB/c x C57Bl/6)F1 female spleen. Myeloma: P3x63Ag8.653. Reactivity: Anti-HLA-DQ. technique(s): cell culture | mammalian: suitable. isotype: IgM. shipped in: dry ice. storage temp.: −. 196°C.- UPC:
- 51314203
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 99043005-1VL
