Cell Line Origin
Mouse x Mouse hybridoma
Cell Line Description
The cell line Lu2 has been established by fusion of the myeloma P3x63Ag8.653 and spleen cells from BALB/c x C57Bl/6 female mice. Mice received a single i.p. injection with the B cell line LuMar expressing HLA A2.3/B18.5/C5/DR3,11.and B. pertussis three days before fusion. The cell line has been eradicated from mycoplasma using Cibrobay.
Immunogen
B cell line LuMar
Application
Immunofluorescence, cytotoxicity, immunoprecipitation
Culture Medium
RPMI 1640 + 2mM glutamine + 10% FBS
Subculture Routine
Maintain cultures between 3-9 x 100,000 cells/ml; 5% CO2; 37°C
Other Notes
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biological source: Mouse x Mouse Hybridoma. ImmunoDon: BALB/c x C57 Bl/6. Myeloma: P3x63Ag8.653. Reactivity: Anti-DR11. technique(s): cell culture | mammalian: suitable. isotype: IgM. shipped in: dry ice. storage temp.: −. 196°C.- UPC:
- 51314203
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 99043003-1VL