Anti-HSF 1 Antibody (C15-1316-848)

General description

HSF1, also known as Heat shock factor protein 1 (HSF-1) or Heat shock transcription factor 1 (HSTF-1), and encoded by the gene HSF1/HSTF1, is a DNA binding transcription factor that binds heat shock promoter elements (HSE’s) and activates transcription. In higher eukaryotes, HSF1 is unable to bind to HSE regions unless the cells are heat shocked. HSF1 is normally a monomer that interacts with HSP90 and other chaperones in a large complex in the cytoplasm. Upon heat shock HSF1 is released from the complex, dimerizes, and is transported into the nucleus. HSF1 is localized to the cytoplasm during normal growth the protein localizes to the nucleus upon heat shock. HSF1 is part of the cellular stress response, and HSF1 is known to interact with many other stress- induced proteins including NCOA6, SYMPK, RALBP1, HSF2, CEBPB, HSPA1A, Heat shock protein 90kDa alpha and HSPA.

Immunogen

Recombinant protein corresponding to mouse HSF 1.

Application

Anti-HSF 1 antibody is an antibody against HSF 1 for use in western blotting, IP & ChIP.

Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated HSF 1 from HEK293 cell lysates transfected with wild type or mutant hRP1-HA (Fujimoto, M., et al. (2012). Mol Cell. 48(2):182-194.).

Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated HSF 1 from hHSF1 transfected MEF cell lysate (Fujimoto, M., et al. (2010). Mol Cell Biol. 21(1):106-116.).

Chromatin Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated HSF 1 from wild type and HSF2-null primary MEF cell lysates (Shinkawa, T., et al. (2011). Mol Cell Biol. 22(19):3571-3583.).

Chromatin Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated HSF 1 from HeLa and MEF cell lysates which were either untreated or heat shock treated (Prof. A. Nakai, Yamaguchi University School of Medicine).

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Transcription Factors

Quality

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting: A 1:1,000 dilution of this antibody detected HSF 1 in 10 µg of HeLa cell lysate.

Target description

~75 kDa observed. Uncharacterized band(s) may be observed in some cell lysates.

Physical form

Rabbit polyclonal serum containing 0.05% sodium azide.

Unpurified

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. Quality Level: 100. antibody form: serum. antibody product type: primary antibodies. clone: polyclonal. species reactivity: human, mouse. technique(s): ChIP: suitable, immunoprecipitation (IP): suitable, western blot: suitable. NCBI accession no.: NP_032322. UniProt accession no.: P38532. shipped in: wet ice. target post-translational modification: unmodified. Gene Information: human ... HSF1(3297). Storage Class Code: 10 - Combustible liquids. WGK: WGK 1.