Anti-Hsp47 Mouse mAb (M16.10A1) (C15-1303-152)

General description

Anti-Hsp47, mouse monoclonal, clone M16.10A1, recognizes the ~47 kDa Hsp47 protein in wide variety of species. It is validated for use in ELISA, EIA, WB, ICC, IF, IP, and IHC (paraffin sections).

Protein G purified mouse monoclonal antibody. Recognizes the ~47 kDa Hsp47 protein.

Recognizes the ~47 kDa Hsp47 protein.

Immunogen

Hsp47 from L6 cells

Rat

Application

ELISA (see comments)

Enzyme Immunoassay (see comments)

Immunoblotting (1 µg/ml, colorimetric)

Immunocytochemistry (1 µg/ml)

Immunofluorescence (5 µg/ml)

Immunoprecipitation (5 µg/ml)

Paraffin Sections (see comments)

Packaging

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Standard Handling (A)

Physical form

In PBS, 50% glycerol, pH 7.2.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Analysis Note

Positive Control
3T3 cells, Human breast cancer tissue

Other Notes

Kuroda, K., et al. 1998. J. Invest. Dermatol.111, 1023.
Nandan, D., et al. 1998. Exp. Cell Res.179, 289.
Clark, E.P., et al. 1991. J. Biol. Chem.266, 17230.
Vaillancourt, J.P., and Cates, G.A. 1991. Biochem. J.274, 793.
Cates, G.A., et al. 1987. Biochem. Cell Biol. 65, 767.
Saga, S., et al. 1987. J. Cell Biol.105, 517.
Nagata, K., et al. 1986. J. Biol. Chem. 261, 7531.

This antibody has also been reported to work for ELISA and enzyme immunoassay. Variables associated with assay conditions will dictate the optimal dilution.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

biological source: mouse. Quality Level: 100. antibody form: purified antibody. antibody product type: primary antibodies. clone: M16.10A1, monoclonal. form: liquid. contains: ≤. 0.1% sodium azide as preservative. species reactivity: mouse, porcine, chicken, canine, human, hamster, guinea pig, bovine, rat, monkey, sheep. manufacturer/tradename: Calbiochem^®. . storage condition: OK to freeze, avoid repeated freeze/thaw cycles. isotype: IgG2b. shipped in: wet ice. storage temp.: −. 20°C. target post-translational modification: unmodified. Gene Information: human ... SERPINH1(871). Storage Class Code: 10 - Combustible liquids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.