Anti-Hsp90 Mouse mAb (AC88) (C15-1303-154)

General description

Protein G purified mouse monoclonal antibody. Recognizes the ~90 kDa Hsp90 protein.

Recognizes the ~90 kDa Hsp90 protein.

This Anti-Hsp90 Mouse mAb (AC88) is validated for use in Immunoblotting, Immunoprecipitation, Immunocytochemistry for the detection of Hsp90.

Immunogen

Achyla ambisexualis

Hsp90 isolated from water mold, Achyla ambisexualis

Application

Immunoblotting (1 µg/ml, colorimetric)

Immunoprecipitation (see comments)

Immunocytochemistry (see comments)

Packaging

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Standard Handling (A)

Physical form

In 10 mM PBS, 50% glycerol, pH 7.2.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Analysis Note

Positive Control
Heat shocked HeLa cells, Heat shocked 3T3 cells, Heat shocked PC-12 cells, Heat shocked CHO-K1, Heat shocked Rat-2 cells

Other Notes

Cross-reacts with glucocorticoid receptor in rodent. By immunoprecipitation, the antibody favors free Hsp90 over complexed Hsp90. The optimal dilution for immunoprecipitation is assay dependent and must be determined for each sample. This antibody has also been reported to work for immunocytochemistry. Variables associated with assay conditions will dictate the optimal dilution.

Harry, J.L. et al. 1990. Development190, 305.
Minoo, P. et al. 1989 J. Cell. Biol.109, 1937.
Riehl, R.M., et al. 1985. Biochemistry 24, 6586.
Sanchez, E.R., et al. 1985. J. Biol. Chem. 260, 12398.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

biological source: mouse. Quality Level: 100. antibody form: purified antibody. antibody product type: primary antibodies. clone: AC88, monoclonal. form: liquid. contains: ≤. 0.1% sodium azide as preservative. species reactivity (predicted by homology): all. manufacturer/tradename: Calbiochem^®. . storage condition: OK to freeze, avoid repeated freeze/thaw cycles. isotype: IgG1. shipped in: wet ice. storage temp.: −. 20°C. target post-translational modification: unmodified. Gene Information: human ... HSP90AA1(3320). Storage Class Code: 10 - Combustible liquids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.