General description
Hu proteins are mammalian embryonic lethal abnormal visual system (ELAV)-like neuronal RNA-binding proteins that contain three RNA recognition motifs. RNA binding protein HuC promotes neuronal development in both the central and the peripheral nervous systems. HuC antibodies are used as markers for developing neurons. HuD may play a role in neuron-specific RNA processing, and be involved in maintaining the proliferative state of cells.
Immunogen
KLH-conjugated linear peptide corresponding to human HuC/HuD.
Application
Immunohistochemistry Analysis: A 1:2,000 dilution from a representative lot detected HuC/HuD in human brain and rat hippocampus tissues.
Research Category
Neuroscience
Research Sub Category
Developmental Signaling
This HuC/HuD antibody is validated for use in WB & IHC for the detection of the HuC/HuD protein.
Quality
Evaluated by Western Blotting in human brain tissue lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected HuC/HuD in 200 µg of human brain tissue lysate.
Target description
~ 40 kDa observed. An uncharacterized band may appear at ~45 kDa in some lysates.
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
Human brain tissue lysate
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Shipping Information:
Dry Ice Surcharge & Ice Pack Shipments: $40
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- UPC:
- 51142501
- Condition:
- New
- Availability:
- 3-5 Days
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- MPN:
- MABN153
- Temperature Control Device:
- Yes
